一株基因Ⅳ型禽呼肠孤病毒的分离与鉴定OA
Isolation and Identification of a Genotype-Ⅳ Avian Reovirus
为了解国内禽呼肠孤病毒病原特性,将临床采集的禽呼肠孤病毒阳性病料研磨后接种鸡胚,经3 次传代后获得1 株禽呼肠孤目标毒株,经鉴定,该株为禽呼肠孤基因Ⅳ型,无其他外源病毒污染,其病毒含量为5×105.5 ELD50/mL.该分离株对鸡胚和SPF鸡均具有明显致病性,可引起接种鸡胚100%死亡,接种3 周龄SPF鸡可引起100%发病.该毒株的成功分离为我国禽呼肠孤病毒毒株流行病学研究提供重要依据.
In order to investigate the pathogenic characteristics of domestic avian reovirus(ARV),the avian reovirus-positive clinical samples were homogenized and then inoculated into chicken embryos.After three passages,a target strain of avian reovirus was obtained.Upon identification,this strain was determined to be ARV genotype Ⅳ,free from contamination by other exogenous viruses,with a viral titer of 5×105.5 ELD50/mL.This isolate exhibited significant pathogenicity towards both chicken embryos and specific-pathogen-free(SPF)chickens,causing 100%mortality in inoculated chicken embryos and 100%morbidity in 3-week-old SPF chickens.The successful isolation of this strain has provided an important basis for epidemiological studies on avian reovirus strains in China.
付博凡;郭思凡;王灵灵;张敏;杨承槐
中国兽医药品监察所(农业农村部兽药评审中心),北京 100081中国兽医药品监察所(农业农村部兽药评审中心),北京 100081中国兽医药品监察所(农业农村部兽药评审中心),北京 100081中国兽医药品监察所(农业农村部兽药评审中心),北京 100081中国兽医药品监察所(农业农村部兽药评审中心),北京 100081
农业科技
禽呼肠孤病毒基因Ⅳ型致病性外源病毒检测病毒含量
avian reovirusgenotype Ⅳpathogenicityexogenous virus detectionviral titer
《中国兽药杂志》 2026 (3)
45-50,6
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