一测多评法同时测定双黄连可溶性粉中4种成分的含量OA
Simultaneous Determination of Four Constituents in Shuanghuanglian Soluble Powder by QAMS
建立双黄连可溶性粉中绿原酸、连翘酯苷A、黄芩苷和黄芩素4 种成分含量的一测多评方法.以黄芩苷为内参物,建立绿原酸、连翘酯苷A和黄芩素的相对校正因子,分别采用外标法与一测多评法测定4 种成分的含量,比较两种方法测得结果的差异,验证一测多评方法的可行性.绿原酸、连翘酯苷A和黄芩素的相对校正因子分别为 1.477、0.869 和 1.513,一测多评法与外标法测定结果无显著性差异,相对偏差小于0.9%.该方法简便、准确、专属性好,可用于双黄连可溶性粉中绿原酸、连翘酯苷A、黄芩苷和黄芩素的质量控制.
To establish a method of quantitative analysis of multi-components by single marker(QAMS)for the determination and evaluation of four constituents(Chlorogenic Acid,Forsythoside A,Baicalin and Baicalein)in Shuanghuanglian Soluble Powder.Baicalin was used as internal reference substance,and the relative correction factors(RCF)of Chlorogenic Acid,Forsythoside A,and Baicalein to Baicalin were calculated and evaluated.The content of the four components were determined by the external standard method(ESM)and QAMS,respectively.The difference between the two methods was compared to verify the feasibility of the method.The RCF of Chlorogenic Acid,Forsythoside A,and Baicalein to Baicalin were 1.477,0.869 and 1.513,respectively.There was no significant differences between the results of external standard method and QAMS,and the relative error was less than 0.9%.The method is simple and accurate with high specificity,which can be used to determine the content of Chlorogenic Acid,Forsythoside A,Baicalin and Baicalein in Shuanghuanglian Soluble Powder.
郭炳恒;杨会鲜;赵志萌;杨世腾;赵涵颖;陈航
鲁山县农业农村局,河南 平顶山 467300洛阳中科基因检测诊断中心有限公司,河南 洛阳 471000洛阳中科基因检测诊断中心有限公司,河南 洛阳 471000洛阳中科基因检测诊断中心有限公司,河南 洛阳 471000洛阳中科基因检测诊断中心有限公司,河南 洛阳 471000新安县动物疫病预防控制中心,河南 洛阳 471000
农业科技
一测多评法高效液相色谱法双黄连可溶性粉绿原酸连翘酯苷A黄芩苷黄芩素
QAMSHPLCShuanghuanglian soluble powderchlorogenic acidforsythoside Abaicalin and baicalein
《中国兽药杂志》 2026 (3)
22-30,9
2019年度洛阳市科技重大专项(1901024A)
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