二倍体和四倍体南川百合差异性状的转录组分析OA

To investigate the changes of Lilium rosthorii homologous tetraploid with diploid,the contents of starch and soluble sugar in leaves of two kinds of ploidies of L.rosthorii were determined,and transcriptome sequencing analysis were performed.The results showed that the contents of soluble sugar and starch in leaves of the homologous tetraploid of L.rosthorii were significantly higher than those of the diploid,and the differences were significant with the extension of growth and development process.Compared with diploid,a total of 20 441 differentially expressed genes(DEGs)were found in tetraploid plants,among which 5 006 DEGs were annotated in GO(gene ontology)database,mainly enriched in plant growth,signal transduction regulation and other functions,and KEGG(Kyoto encyclopedia of gene and genomes)pathway analysis revealed significant differences in differential genes in pathways such as foreign substance metabolism,zeaxanthin synthesis and aminoacyl tRNA synthesis.In addition,7 DEGs related to glucose metabolism were screened and enriched in UDP glucosyltransferase activity and O-glycosyl compound hydrolase activity,and 10 DEGs related to pathogen response were screened and enriched in plant pathogen interactions and plant MAPK signaling pathway.Above results provided data foundation for in-depth research on gene function,also provided theoretical basis and data support for promoting the polyploidization process of lilies and cultivating excellent germplasm resources.