陆地棉GhABC1K3-A05基因响应干旱、温度胁迫的功能分析OA

ABC1K(activity of bc1 complex kinase)gene family encodes a kind of atypical protein kinases,which plays an important role in plant growth and development and response to stress.The transcriptome analysis of abiotic stress in upland cotton showed that the expression patterns of 40 GhABC1K family genes were different,and GhABC1K3-A05 strongly responded to 3 kinds of abiotic stress.To clarify the function of GhABC1K3-A05 in response to abiotic stress,virus-induced gene silencing(VIGS)technique was used to silence GhABC1K3-A05 in upland cotton.The results showed that,after drought stress,the leaf water loss rate of GhABC1K3-A05 silenced plants increased,the relative water content of leaves decreased significantly,the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)significantly decreased,and the content of malonaldehyde(MDA)significantly increased,the chlorophyll content significantly decreased compared with the empty carrier control plants.Under high temperature stress,the SOD and CAT activities of GhABC1K3-A05 silenced plants significantly decreased,the MDA content significantly increased,and the chlorophyll content significantly decreased compared with the control plants.After low temperature stress,SOD,POD and CAT activities of GhABC1K3-A05 silenced plants decreased significantly,the MDA content increased,and the chlorophyll content significantly decreased compared with the control.To sum up,GhABC1K3-A05 gene played an important role in the response of cotton to abiotic stress,which provided available genetic resources for anti-stress biological breeding of upland cotton.