黄淮麦区新育小麦品种(系)LMW-GS组成与品质评价OA

To clarify the grain quality and genetic diversity of low-molecular-weight glutenin subunits(LMW-GS)in newly bred wheat varieties(lines)at the Huanghuai wheat region,84 newly approved or under trial wheat varieties(lines)were used as materials.The composition and frequency distribution of LMW-GS were detected using 22 pairs of specific primers,and cluster analysis was conducted in combination with 6 grain quality indicators.The results showed that a total of 9 LMW-GS alleles were detected.At the Glu-A3 locus,3 alleles were detected including Glu-A3d(58.33%),Glu-A3a/c(47.62%)and Glu-A3a(4.76%).At the Glu-B3 locus,only the Glu-B3d allele was detected,with a frequency of 29.76%.At the Glu-D3 locus,Glu-D3-21(69.05%),D3-32(58.33%),D3-23(41.67%),D3-31(2.38%)and D3-22(1.19%)alleles were detected.Both A3a/c and B3d showed a significant positive correlation with wet gluten content.A total of 40 LMW-GS combination types were identified,and the freqnencies of A3d+D3-21(17.86%),A3d+D3-23+D3-32(7.14%)combinations were high.Based on grain quality traits,84 varieties(lines)were clustered into 4 groups.Group Ⅱ exhibited superior comprehensive quality characteristics.Group Ⅳ included multiple strong-gluten or medium-strong-gluten varieties,such as Xinmai 26,Xinong 979 and Zhengmai 366,etc.Above results provided important theoretical reference for wheat quality breeding.