当归黄芪超滤物对电离辐射诱导下心肌细胞的影响及其机制研究OA

AIM:To investigate the effects of ion-izing radiation on H9C2 cardiomyocytes and the in-tervention efficacy of Radix Angelicae Sinensis-Radix Astragali ultrafiltrate(RAS-RH).METHODS:An H9C2 cardiomyocyte injury model was established using X-ray irradiation,followed by intervention with RAS-RH.Cell viability was assessed using the CCK-8 assay.Phalloidin staining was employed to observe changes in the cytoskeletal structure of car-diomyocytes.JC-1 staining combined with flow cy-tometry was used to measure mitochondrial mem-brane potential(ΔΨm).Hoechst 33 324 staining and flow cytometry were applied to evaluate apoptosis.Western blot was performed to determine the ex-pression levels of Drp1 and HSP70 proteins.RE-SULTS:(1)X-ray irradiation significantly inhibited the proliferation of H9C2 cells(P<0.05),disrupted cytoskeletal integrity,reduced cell viability,altered mitochondrial membrane potential(ΔΨm),and promoted apoptosis(P<0.01,P<0.05).(2)Interven-tion with RAS-RH(Radix Astragali and Radix Angeli-cae Sinensis ultrafiltrate)markedly enhanced H9C2 cell viability(P<0.01),restored cytoskeletal organi-zation,and improved proliferative capacity,ΔΨm,and apoptosis resistance(P<0.01,P<0.05).(3)Mechanistically,RAS-RH ameliorated H9C2 cell function by modulating the relative protein expres-sion levels of Drp1(Dynamin-related protein 1)and HSP70(Heat shock protein 70)(P<0.05).CONCLU-SION:Ionizing radiation impairs the biological func-tion of H9C2 cardiomyocytes by disrupting the cy-toskeletal structure,reducing cell viability,down-regulating ΔΨm,and promoting apoptosis.In con-trast,RAS-RH can mitigate radiation-induced car-diomyocyte damage by regulating the relative ex-pression levels of Drp1 and HSP70 proteins.