阿司匹林在缺血性脑卒中患者中的基因多态性临床研究OA

Objective To analyze the gene polymorphism of aspirin drug-related genes platelet membrane glycoprotein Ⅲa phospholipase A2(GPⅢa PLA2),platelet endothelial aggregation receptor 1(PEAR1),glutathione S-transferase P1(GSTP1)and prostaglandin endoperoxide synthase 1(PTGS1)in patients with ischemic stroke in Beijing.Methods Patients with ischemic stroke in the neurology department of our hospital were included as research subjects,and the polymorphism of drug-related gene loci was detected by fluorescence in situ hybridization sequencing.Results The overall mutation rate of aspirin related genes was 75.42％(356 cases/472 cases).GPⅢa PLA 2 typing included 458 cases TT type,11 cases TC type and 3 cases CC type,C allele frequency was 1.80％(17 cases/944 cases);PEAR1 typing included 187 cases GG type,224 cases GA type and 61 cases AA type,A allele frequency was 36.65％(346 cases/944 cases);GSTP1 typing included 295 cases AA type,160 cases GA type and 17 cases GG type,G allele frequency was 20.55％(194 cases/944 cases);typing of PTGS1 included 50 cases AA type,and the frequency of G allele was 0.GPⅢa PLA2 TT+PEAR1 GA+GSTP1 AA was the most common combination,accounting for 138 cases(29.24％).There was no statistically significant difference in gene polymorphisms of GPⅢa PLA2,PEAR1,GSTP1 between different genders and ages(all P＞0.05).The genotype and allele distributions of GPⅢa PLA2,PEAR1,GSTP1 and PTGS1 in patients with ischemic stroke in Beijing were not statistically significantly different from those in Zaozhuang,Nanjing and Shanghai(all P＞0.05),while the genotype and allele distributions of GPⅢa PLA2,PEAR1 and GSTP1 were statistically significantly different from those in Guangdong(all P＜0.05).Conclusion PEAR1 and GSTP1 are the main mutations in aspirin drug-related genes in patients with ischemic stroke in Beijing.The mutation ratio of GPⅢa PLA2 and PTGS1 gene is low,and the gene polymorphism in Beijing is different from that in other regions.