红阳猕猴桃核酮糖-1,5-双磷酸羧化酶/加氧酶小亚基基因的克隆与表达分析OA

Ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)plays a crucial role in plant photosynthesis,and its high expression is closely related to photosynthetic efficiency.Cloning the gene encoding the small subunit of ribulose-1,5-bi-sphosphate carboxylase/oxygenase(RuBisCo)in Hongyang kiwifruit(Actinidia chinensis'Hongyang'),and analyzing its expression pattern and biological function can provide a theoretical basis for targeted breeding strategies aimed at enhancing the photosynthetic productivity of Hongyang kiwifruit.The AcRbcS gene,a small subunit gene,was cloned from Hongyang kiwifruit leaf.Its expression profile in different leaf development stages was investigated using RNA-seq and real-time quantitative PCR(qPCR).The results showed that the full-length coding sequence(CDS)of AcRbcS was 369 bp,encoding a protein of 122 amino acids with a molecular weight of 13.75 kDa.The expression level of AcRbcS increased progressively during leaf development,reaching a peak at the light-green stage.This study provided valuable insights for future molecular breeding efforts to improve the photosynthetic efficiency and fruit quality of Hongyang kiwifruit.