黔东南38份树莓资源的遗传多样性分析OA

To explore the genetic relationships and determine the genetic distances among raspberry resources in Qiandongnan,so as to provide a scientific basis for elite cultivar identification,variety breeding,and rational parent selection,a total of 38 raspberry accessions from Qiandongnan Prefecture were used as materials to analyze their genetic diversity using sequence-related amplified polymorphism(SRAP)molecular marker technology.The results showed that 10 polymorphic primer combinations were selected from 80 primer pairs,and the polymorphism rate of the bands amplified by these 10 primer combinations was 96.20％.The 38 wild and cultivated raspberry accessions were divided into two populations,with an observed allele number of 1.9624 and an effective allele number of 1.335 1.At the same time,the Nei's gene diversity index was 0.219 6 and the Shannon information index was 0.356 2.The effective gene number,gene diversity index(H),and Shan-non's information index(Ⅰ)values of wild raspberry resources were all lower than those of cultivated varieties.The results of cluster analysis showed that cultivated varieties and wild germplasm are not clustered separately,and the genetic distance between Rubus coreanus(Miq.)Miq.,and the other 37 accessions was relatively large.SRAP molecular markers are highly valuable for studying the genetic diversity of raspberries.Future research should focus on in-depth genetic studies of R.coreanus to provide more accurate information for raspberry breeding programs.