东莞地区献血者血小板基因型资料库容量分析OA

Objective To establish a database of platelet human leukocyte antigen(HLA)and human platelet antigen(HPA)genotypes for blood donors in Dongguan City,and estimate the appropriate capacity of the database,so as to pro-vide a guarantee for clinical platelet transfusion therapy.Methods HPA-1-15 and HLA-A,B genotyping was performed on 200 blood samples included in the platelet gene bank construction standard of blood donors in this station by three-generation gene sequencing technology.The allele frequency and haplotype frequency of HPA-1-15 and HLA-A,B were statistically an-alyzed,and the probability of finding at least one HLA and HPA matching blood donor and the appropriate bank capacity were calculated in turn.Results HPA-1-15 and HLA-A,B were polymorphic in this region.According to the frequency of haplotypes,it was estimated that when the platelet donor pool capacity was 713 people,the patients with haplotype frequency>0.0025 had a 95%probability of finding at least one HPA-1-15 matched blood donor in the gene pool.The establishment of a platelet donor gene pool with a pool of 2381 people could make the patients with haplotype≥0.0025 had a 90%proba-bility of finding at least one HLA-Ⅰ-A,and B matched blood donor.If it was necessary to fully cooperate with HLA and HPA at the same time,the pool capacity must be at least 7721 people.Conclusion The estimation of the capacity of the platelet donor database directly affects the effectiveness of its establishment.Dongguan has already established a database of HLA and HPA genotypes for 200 donors,but it needs to continue expanding the capacity of the database.