基于血管生成拟态相关基因的肝细胞癌预后模型构建OA

Objective To construct a prognostic model for hepatocellular carcinoma(HCC)based on vasculogenic mim-icry(VM)related genes.Methods HCC patient data were retrieved from The Cancer Genome Atlas(TCGA)database.Correlation between ALYREF and HCC prognosis was assessed through survival analysis,receiver operating characteristic(ROC)curves,and calibration curves.Gene set enrichment analysis(GSEA)was employed to investigate association between ALYREF and VM related pathways.Univariate Cox regression,least absolute shrinkage and selection operator(LASSO)regression,and random forest(RF)algorithm were performed to screen ALYREF-based VM related key genes.An ALYREF-related vasculogenic mimicry prognostic model(AVMP)was constructed and the risk score(AVMP-Risk Score,AVMP-RS)was calculated.Univariate and multivariate Cox regression analysis were conducted to determine whether AVMP-RS was an independent prognostic factor.The performance of AVMP was evaluated using ROC curves,calibration curves,and nomograms.Finally,the HCC-LIHC dataset from the ICGC database was used as an external validation set for verification.Results The expression level of ALYREF was significantly elevated in HCC patients at higher stages(Stage)and grades(Grade).The prognosis of patients in the High-ALYREF group was poor,and its expression levels had high ac-curacy in predicting the 1-year,2-year,and 3-year survival rates of patients.GSEA analysis showed that DEGs between the High-ALYREF group and Low-ALYREF group were significantly enriched in classic VM pathways such as TGF-B1,HIF1A,PI3K/AKT/mTOR,Notch,Wnt,and VEGFA/VEGFR2.91 ALYREF related VM genes were obtained,and LASSO regres-sion combined with RF algorithm was used to screen KIF20A and CBX2 genes for constructing the prognostic model AVMP.Univariate and multivariate Cox regression analysis showed that AVMP-RS was an independent risk factor for the prognosis of HCC patients,and its predictive accuracy was superior to other clinical factors such as age,gender,staging,and grading.Column charts could effectively predict the survival rate of patients within 3 years.External data validation indicated that AVMP had good applicability.Conclusion This study successfully constructs a VM related gene HCC prognosis model AVMP based on ALYREF,which shows high accuracy in predicting the prognosis of HCC patients and provides important reference for identifying high-risk patients and evaluating prognosis.