决明子提取物和多烯磷脂酰胆碱对小鼠酒精性肝损伤的影响OA

Objective To investigate the intervention effects of Cassia seed extract(CSE)and polyene phosphatidylcholine(PPC)on alcohol liver injury in mice.Methods 40 C57BL/6J mice were randomly divided into blank control group,model control group,CSE group,PPC group,and CSE-PPC combination group.A liver injury model in C57BL/6J mice was estab-lished using the NIAAA model(10 days of alcohol liquid feed+single high-dose alcohol gavage),and mice were subsequently treated with CSE(2 g·kg-1·d-1),PPC(200 mg·kg-1·d-1),and a combination of both,respectively.Serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglycerides(TG),and hepatic superoxide dismutase(SOD)levels were analyzed,and liver pathological changes were also evaluated.Results Compared with the blank control group,the model control group mice showed significantly reduced food intake(P<0.001)and body weight(P<0.05),as well as marked-ly elevated liver index(P<0.01).Serum ALT(P<0.001),AST(P<0.05),and TG(P<0.01)levels significantly in-creased,while liver tissue SOD levels decreased,and hepatic steatosis was pronounced.After intervention,the levels of ALT(P<0.001)and TG(P<0.05)significantly reduced in the PPC group;the ALT level decreased(P<0.05)and SOD activity increased(P<0.01)in the CSE group;the combined group(n=6)showed a reduction in ALT and AST(P<0.05)compared to the CSE group,SOD activity showed a trend of increase,food intake improved,and pathology showed the most significant improvement.Conclusion CSE and PPC can effectively ameliorate alcohol liver injury through synergistic regulation of oxida-tive stress and lipid metabolism.The combined use of these drugs yields better results than single intervention,which provides a new strategy for the prevention and treatment of alcoholic liver disease.