宏基因二代测序对不明原因宫内感染的病原学诊断价值OA

Objective To investigate the diagnostic efficacy of metagenomic next-generation sequencing for unexplained intrauterine infection,and to clarify its diagnostic value and clinical significance.Methods Two patients with unexplained intrauterine infection(infection group)and one control case(control group)admitted to Weifang Maternal and Child Health Hospital from January to July 2024 were selected.The clinical information of the patients was collected,and the differences in positive rate and pathogen distribution between traditional detection methods and metagenomic sequencing were compared.After DNA extraction of the samples,the library was constructed after quality control,and the data were obtained by high-throughput sequencing.The sequencing data were annotated by Kraken2 software and compared with UHGG and NCBI database for the classification and identification of pathogenic microorganisms,and the bioinformatics depth analysis was carried out by KEGG.Results The positive rate of metagenomic sequencing in pathogen detection and bacterial infection detection was higher than that of traditional detection methods(P<0.05),but there was no difference in the detection of chlamydia,virus,mycoplasma and fungal infection(P>0.05).The types of pathogens in the infection group were less than those in the control group,and there were 10 unique pathogens in the infection group(such as Corynebacterium tuberculosis,Lactobacillus johnsonii).Enrichment analysis showed that the genes were mainly concentrated in ribosome-related pathways.GSEA analysis showed that MRPS12 and MRPL23 played an important role in retinol metabolism,cytochrome metabolism and immune-related pathways.Conclusion Compared with traditional detection,metagenomic detection has better diagnostic performance in patients with unexplained intrauterine infection,especially in rare or difficult to cultivate pathogens.