新疆南美白对虾副溶血性弧菌的分离鉴定OA

In order to identify infection status of Vibrio parahaemolyticus(V.parahaemolyticus)in Litopenaeus vannamei(L.vannamei)in major farming areas of Xinjiang,187 shrimp samples(including post-larvae,juveniles and adults)were collected from 47 ponds where disease outbreaks were suspected on 12 farms in major production areas of Bayingolin,Changji and Tacheng from June to September 2025,to isolate and purify vibrio species using TCBS agar medium,and to identify bacterial strains through 16S rDNA sequence analysis;concurrently,DNAwas extracted from hepatopancreas,hepatopancreas enrichment broth and the isolated strain for PCR assay to compare and analyze the test results.The results showed that the overall detection rate of V.parahaemolyticus was 21.4％(40/187),with a total of 73 strains isolated;over 99％sequence homology was observed between the isolated strain and the standard strain,attesting that the identification was accurate;based on the results of different samples,the detection rate by PCR was the highest for isolated strain(21.4％),followed by hepatopancreas enrichment broth(19.8％),and then hepatopancreas(9.6％).In conclusion,V.parahaemolyticus infection was available in L.vannamei in Xinjiang;and DNA extracted from hepatopancreas enrichment broth was recommended for PCR assay for samples with low bacterial loads and in rapid screening scenarios.