miR-20a-5p在民猪前脂肪细胞分化和产热中的作用OA

[Objective]This study investigated the expression of miR-20a-5p in various tissues and fat deposits in different parts of the Min pig,as well as its influence on the differentiation of pre-adipocytes and thermogenesis-related genes,to elucidate its role in the development of cold resistance traits in Min pigs.[Method]The expression levels of miR-20a-5p were compared across seven tissues and organs(the heart,liver,spleen,lungs,kidneys,back muscle,and back fat)and the fat tissues from six distinct body parts(back,abdomen,neck,buttocks,chest,and inguinal area)under varying temperature conditions,with RT-qPCR as the tool.Furthermore,miR-20a-5p was overexpressed in pre-adipocytes cultured in vitro from Min pigs to assess its impact on pre-adipocyte differentiation and key gene expression in a significant ther-mogenic pathway.[Result]The expression of miR-20a-5p was significantly higher in the liver and back muscles compared to other organs,while its expression in chest fat was extremely significantly higher than that in fat from other parts.After Min pigs were subjected to cold stimulation,there was a significant in-crease in the expression of miR-20a-5p in the fat of the back and neck of Min pigs,and a significant de-crease in the inguinal fat.Overexpression of miR-20a-5p in pre-adipocytes cultured in vitro significantly en-hanced lipid droplet formation and beige adipocyte differentiation-related gene expression,significantly in-hibited the UCP3 pathway thermogenesis-related gene expression,and increased the expression of key genes of the Ca2+cycling pathway and the creatine futile cycle pathway.[Conclusion]miR-20a-5p is ubi-quitously expressed across various tissues and organs in pigs.However,its expression levels demonstrate significant difference.When pigs are subjected to cold stimulation,the miR-20a-5p expression in fat tissues from different parts exhibits difference in their expression levels.In Min pigs,miR-20a-5p can induce the beige of preadipocytes,impede heat production via the UCP3 pathway,and concurrently,enhance heat pro-duction through both the Ca2+cycling and creatine futile cycle pathways.