电针联合膈下逐瘀汤加减方对气滞血瘀型慢性输卵管炎大鼠的影响OA

Objective It is to investigate the effect and mechanism of electroacupuncture(EA)combined with modified Decoction for dissipating bold stasis under diaphragm(DDBSUD)on chronic salpingitis with syndrome of Qi stagnation and blood stasis in rats.Methods Forty-eight female SD rats were randomly divided into normal group,model group,DDBSUD group,EA+DDBSUD group,with 12 rats in each group.The rats in the control group were not treated,while the rats in the other groups were prepared to establish models of chronic salpingitis with syndrome of Qi stagnation and blood stasis.On the 29th day of the experiment,the model group was administered purified water by gavage,the DDBSUD group was adminis-tered 1.50 g/mL suspension of DDBSUD by gavage,and the EA+DDBSUD group were treated with both EA and 1.50 g/mL suspension of DDBSUD by gavage,all administered once daily,continuously treated for 4 weeks.The scores of syndrome of Qi stagnation and blood stasis of the rats were compared among the groups,the histopathological morphology of fallopian tube tissue was observed by HE staining,the contents of type Ⅰ collagen(Col-Ⅰ),tissue inhibitor of metalloproteinases-1(TIMP-1),matrix metalloproteinase-9(MMP-9)and the MMP-9/TIMP-1 ratio in fallopian tube tissues were measured by ELISA,the positive expressions of MMP-9 and TIMP-1 in fallopian tube tissues were detected by immunohistochemistry,and the expression of Col-Ⅰ mRNA in serum was detected by RT-qPCR.Results Compared with the control group,the to-tal score of syndrome of Qi stagnation and blood stasis of rats in the model group was significantly increased(P<0.05);significant inflammatory damage was observed in the fallopian tubes;the contents of Col-Ⅰ and TIMP-1,the mean optical density of TIMP-1 positive expression in fallopian tube tissue,and the relative expression of Col-Ⅰ mRNA in serum were all significantly increased(all P<0.05),while the content of MMP-9,MMP-9/TIMP-1 ratio,and the mean optical densi-ty of MMP-9 positive expression were all significantly reduced(all P<0.05).Compared with the model group,the total scores of syndrome of Qi stagnation and blood stasis of rats in the DDBSUD group and EA+DDBSUD group were signifi-cantly decreased(both P<0.05);the inflammatory damages in the fallopian tubes were alleviated;the contents of Col-Ⅰand TIMP-1,the mean optical density of TIMP-1 positive expression in fallopian tube tissue,and the relative expression of Col-Ⅰ mRNA in serum were all significantly decreased(all P<0.05),while the contents ofMMP-9,MMP-9/TIMP-1 ra-tios,and the mean optical densities of MMP-9 positive expression were all significantly increased(all P<0.05),and the improvements of these indicators and inflammatory damages in the fallopian tubes in the EA+DDBSUD group were more significant than those in the DDBSUD group(all P<0.05).Conclusion EA combined with DDBSUD can significantly al-leviate inflammatory damage and fibrosis in the fallopian tubes of rats with chronic salpingitis with syndrome of Qi stagnation and blood stasis;the mechanism may be related to inhibition of collagen deposition,regulation of the dynamic balance be-tween MMP-9 and TIMP-1,and the regulation of extracellular matrix metabolism.