磷酸二酯酶3特异性抑制剂米利酮体外对人成熟精子运动能力的影响OA

Objective:To investigate the effects of phosphodiesterase 3(PDE3)specific inhibitor milrinone on the motility of sperm from healthy individuals and asthenozoospermic patients in vitro.Methods:Sperm from healthy individuals and asthenozoospermic patients were treated in vitro with 10 μmol/L milrinone for 1 h.The two types of sperm without milrinone treatment served as the control groups.Sperm motility parameters were assessed using a computer-assisted sperm analysis(CASA)system.Results:Compared with the control,milrinone had no effect on total motility,progressive motility,non-progressive motility and motility parameters in healthy sperm(P＞0.05).Compared with the control in asthenozoospermic samples,milrinone significantly increased the total motility,progressive motility,non-progressive motility,and also enhanced the mean curvilinear velocity,mean straight-line velocity,average path velocity,and mean beat-cross frequency of sperm(P＜0.05).There were no statistically significant differences between the two groups in terms of sperm lateral head displacement and movement patterns(mean amplitude of lateral head displacement,mean angular displacement,linearity,wobble and straightness)(P＞0.05).Conclusion:Milrinone can effectively activate sperm motility in asthenozoospermic patients in vitro.