基于全基因组重测序的"松粳"系列水稻品种及其亲本的遗传关系分析OA

[Objective]The analysis of genetic diversity of elite varieties can provide a deeper understanding of the breeding process and genetic differences among varieties,and provide more valuable diversity data and breeding strategy support for guiding molecular breeding practices.[Methods]This study analyzed the elite rice varieties of the"Songgeng"series and their associated elite parents or derived varieties in the first accumulated temperature zone of Heilongjiang Province through whole genome resequencing technology.A phylogenetic tree was constructed using single nucleotide diversity data and genetic distance was calculated.[Results]A total of 2 908 684 SNP loci and 685 321 InDel loci were obtained through population sequencing.The analysis of SNP differences among varieties showed that there were more than 600 000 SNP differences between Wuyoudao A and Dongnong V4,Songgeng 13,Jigeng 88 and Wuyoudao 4.However,there were only 685 321 SNPs between Songgeng 6 and Wuyoudao 3.The phylogenetic tree show that Wuyoudao 4,Songgeng 22 are grouped together,while Wuyoudao 1,Song 98-131,Songgeng 8,Songgeng 9,and Songgeng 16 are grouped together and have close genetic relationships.The genetic distance analysis shows that the genetic distance between Wuyou A and other varieties is relatively long,with a largest genetic distance of 0.219 1 from Songgeng 13.The genetic distance between Wuyoudao 3 and Songgeng 6 is the closest,only 0.002 6.[Conclusion]Differential analysis or cluster analysis can be used to assess the relationship or genetic distance between individuals.In the formulation of breeding plans,priority should be given to crossing parents with greater genetic distance.This approach helps generate segregating progeny with greater diversity,thereby increasing the probability of selecting superior new varieties.