基于因子分析与聚类分析的帕金森病患者中医证候特征及温肾养肝方对其血清炎症因子和神经营养因子水平的影响OA

Objective To explore the traditional Chinese medicine(TCM)syndrome characteristics of Parkinson's disease(PD)patients and the effects of Wenshen Yanggan Formula on their serum inflammatory and neurotrophic factor levels based on factor analysis and clustering analysis.Methods A retrospective analysis was conducted on 80 PD patients admitted to the First Affiliated Hospital of Guangxi University of Chinese Medicine from December 2021 to November 2023.The patients were divided into a control group and an observation group based on whether Wenshen Yanggan Decoction was included in the treatment,with 40 patients in each group.Then,Kaiser-Meyer-Olkin(KMO)and Bartlett's Test of Sphericity were employed to assess whether the data fulfilled the prerequisites for factor analysis before applying factor and clustering analyses to the PD symptoms collected.Finally,a repeated measure analysis of variance(ANOVA)was conducted to evaluate the efficacy of Wenshen Yanggan Decoction on PD.Results Common symptoms of PD included tremor and bradykinesia.Eight common factors were extracted,involving syndrome elements such as internal wind and yin deficiency.Through clustering analysis,the symptoms were classified into three clusters,namely internal heat and yang hyperactivity,spleen yang deficiency,as well as qi and blood deficiency.The clustering results were clear and consistent with clinical practice.After treatment,the TCM syndrome scores were decreased in both groups(P<0.05),and the scores were significantly lower in the observation group(P<0.05).According to clustering analysis,the TCM syndrome scores were significantly decreased in the patients of the three clusters,with statistically significant differences in the score reductions.Among these clusters,the greatest score reduction was observed in the third cluster,qi and blood deficiency type(P<0.05).The main effect analysis results revealed statistical significance in the time effect and the group effect of serum inflammatory factors and neurotrophic factors(P<0.001),as well as in the interaction effects of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),brain-derived neurotrophic factor(BDNF),and glial-derived neurotrophic factor(GDNF)levels between the two groups(P<0.05).IL-6 and TNF-α levels were decreased,while GDNF and BDNF levels were increased in both groups(P<0.05),and the differences were more significant in the observation group(P<0.05).Conclusion In this study,34 selected symptoms were clustered into 3 categories:internal heat and yang hyperactivity,spleen yang deficiency,along with qi and blood deficiency.After treatment with Wenshen Yanggan Decoction,TCM syndrome scores were decreased significantly,especially for patients with qi and blood deficiency.Additionally,the Decoction effectively lowers serum inflammation levels and promotes neurotrophic factor release.