补肾柔肝汤辅助治疗卵巢储备功能下降肾虚肝郁型患者的临床疗效及对其炎症和卵巢微环境的影响OA

Objective To investigate the clinical efficacy of Bushen Rougan Decoction as an adjunctive therapy for diminished ovarian reserve(DOR)presenting with the kidney deficiency and liver depression pattern,and to examine its effects on inflammation and ovarian microenvironment.Methods Eighty-seven DOR patients treated at the Eye Hospital of China Academy of Chinese Medical Sciences from January 2022 to January 2024 were enrolled and randomly divided into a control group(n=44)and an observation group(n=43)using a random number table.The control group received Femoston treatment,while the observation group received Bushen Rougan Decoction combined with Femoston.After three menstrual cycles of treatment,the clinical efficacy and adverse reactions were compared between the two groups.The assessments included the traditional Chinese medicine(TCM)syndrome scores,laboratory indicators[serum follicle-stimulating hormone(FSH),luteinizing hormone(LH),anti-Müllerian hormone(AMH),growth differentiation factor-9(GDF-9),and Toll-like receptor 2(TLR2)],and ovarian microenvironment status[peak systolic velocity(PSV)of ovarian arteries]measured before and after treatment.Results The total clinical effective rate in the observation group was 95.35%(41/43),which was significantly higher than that in the control group(79.55%,35/44)(P<0.05).After treatment,the TCM syndrome scores in both groups were significantly lower than those before treatment(P<0.05),with the observation group showing significantly lower scores than the control group(P<0.05).After treatment,the levels of FSH and LH in both groups were significantly lower than before treatment,while the AMH levels were significantly higher(P<0.05).Moreover,the FSH and LH levels in the observation group were significantly lower than those in the control group,while the AMH level was significantly higher(P<0.05).After treatment,the TLR2 levels significantly decreased in both groups,while the PSV and serum GDF-9 levels significantly increased in both groups(P<0.05).Additionally,the observation group showed significantly lower TLR2 level and significantly higher PSV and serum GDF-9 level than the control group(P<0.05).No statistically significant difference was observed in the incidence of adverse reactions between the two groups during the treatment(P>0.05).Conclusion As an adjunctive therapy,Bushen Rougan Decoction can improve clinical efficacy,alleviate menstrual abnormalities,regulate sex hormone levels,suppress inflammation,and improve ovarian function and the ovarian microenvironment in DOR patients with the kidney deficiency and liver depression pattern.