睡眠特征与便秘发生风险的遗传关联分析及机制探索OA

Objective:To explore the genetic causal association between six sleep characteristics and constipation using Mendelian randomization analysis.Methods:This study selected the genome-wide association study data of six sleep characteristics from the OpenGWAS database of the Medical Research Council Integrative Epidemiology Unit at University of Bristol and the genome-wide association study data of constipation from the catalog database of Genome Wide Association Study.The inverse variance weighted method(IVW)was adopted as the main analysis method to evaluate the causal relationship between the six sleep characteristics and constipation.MR-Egger and weighted median method(WM)were used as supplementary evidence for the IVW results.At the same time,heterogeneity and horizontal pleiotropy tests were conducted to ensure the stability of the results.The instrumental variables of the positive results were annotated,and enrichment analysis of related genes was performed to predict their potential mechanisms.Results:The IVW method results showed that insomnia increased the risk of constipation(P=0.002),and sleep duration also affected constipation(P=0.026).Sensitivity analysis indicated that the results were stable.Gene enrichment analysis revealed that insomnia may affect the occurrence of constipation through the immune system pathway,while sleep duration may exert its effect through mechanisms such as regulating the intestinal-brain neural function and catecholamine secretion.Conclusions:Insomnia is a risk factor for constipation.Ensuring adequate sleep has guiding significance for the prevention and treatment of constipation.