番茄低温胁迫响应机制与分子育种研究进展OA

The low-temperature tolerance of tomatoes is a key trait that affects their yield and quality,and it is also an important breeding objective under the context of protected cultivation and climate change.However,the molecular regulatory network underlying cold tolerance in tomatoes has not been fully elucidated,and the application of key genes in breeding practices remains limited.To address these issues,this paper systematically reviews the molecular mechanisms of tomato response to low-temperature stress and the progress in breeding applications.At the molecular level,the early perception and transduction processes initiated by changes in membrane fluidity,calcium signaling,reactive oxygen species(ROS)signaling and hormonal signaling(ABA,JA,etc.)are sequentially elaborated.The complex transcriptional regulatory network centered on the ICE1-CBF-COR transcriptional cascade and integrating transcription factors such as NAC,WRKY and bZIP are discussed.Additionally,the fine regulatory mechanisms involving epigenetic factors such as DNA methylation,histone modifications,and non-coding RNAs like miRNAs,lncRNAs and circRNAs,as well as key metabolic pathways and related genes encompassing enzymatic antioxidation,osmotic regulation and membrane lipid remodeling are examined.From a breeding perspective,the paper systematically outlines the exploration of superior alleles in wild germplasm resources and the application strategies and outcomes of molecular technologies,including genetic engineering,gene editing and multi-omics-assisted approaches,in the innovation of cold-tolerant germplasms.Finally,the article summarizes the current challenges in elucidating complex regulatory networks and translating laboratory findings into field applications and provides an outlook on future research directions.It emphasizes that measures such as deepening mechanistic analysis,optimizing technological tools,strengthening germplasm innovation,and promoting industrial application should be implemented to systematically enhance the theoretical framework and practical efficacy of molecular breeding for low-temperature tolerance in tomato.These efforts aim to provide theoretical and technical support for addressing climate challenges and meeting industrial demands.