水溶性金盏花提取物的制备和护肤功效研究OA
Preparation and skincare efficacy of water-soluble calendula officinalis L.extract
使用反相大孔树脂柱层析和膜分离技术,制备水溶性金盏花提取物,并通过DPPH·和ABTS·自由基模型、透明质酸酶抑制实验和细胞划痕实验检测金盏花提取物的抗氧化、舒缓、修复活性.结果显示,5%的金盏花提取物(1 475 μg/mL)对DPPH·和ABTS·自由基的抑制率达到了94.75%和99.35%,具有较好的抗氧化功效;5%的金盏花提取物对透明质酸酶的抑制率达到66.72%,具有较强的舒缓功效;添加1%的金盏花提取物(295 μg/mL)细胞划痕实验中愈合率达到了30.20%,具有较强的修复功效.最后通过HaCaT细胞活力实验验证了金盏花提取物具有良好的安全性.
Water-soluble calendula officinalis L.extract was prepared using reverse-phase macroporous resin column chromatography combined with membrane separation techniques.The antioxidant,soothing,and repair activities of the extract were evaluated using DPPH·and ABTS·radical scavenging assays,hyaluronidase inhibition assay,and cell scratch assay.Results showed that 5%calendula officinalis L.extract(1 475 μg/mL)exhibited inhibition rates of 94.75%and 99.35%against DPPH·and ABTS·radicals,respectively,demonstrating strong antioxidant activity.Additionally,5%calendula officinalis L.extract inhibited hyaluronidase by 66.72%,indicating notable soothing effects.In the cell scratch assay,1%extract(295 μg/mL)achieved a wound healing rate of 30.20%,reflecting substantial repair efficacy.Furthermore,HaCaT cell viability assays confirmed that the calendula officinalis L.extract exhibited good biological safety.
周浩锋;林雨锜;杜倩文;田甲佳;邢新会;王晶
清华大学深圳国际研究生院,广东 深圳 518000||深圳波顿香料有限公司,广东 深圳 518000深圳波顿香料有限公司,广东 深圳 518000深圳波顿香料有限公司,广东 深圳 518000深圳波顿香料有限公司,广东 深圳 518000清华大学深圳国际研究生院,广东 深圳 518000深圳波顿香料有限公司,广东 深圳 518000
化学化工
金盏花提取物抗氧化舒缓修复
calendula officinalis L.extractantioxidantsoothingrepair
《日用化学品科学》 2026 (4)
47-52,64,7
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