GRIN2D在结直肠癌中的表达和意义OA

Objective To investigate the expression and significance of glutamate receptor,ionotropic,N-methyl-D-aspar-tate 2D(GRIN2D)in colorectal cancer(CRC)tissue.Methods Cancerous tissue samples and adjacent control tissue samples were collected from 266 patients with stage Ⅰ—Ⅲ primary CRC who underwent surgical treatment in The Affiliated Hospital of Qingdao University from 2016 to 2018.Immunohistochemistry was used to measure the expression of GRIN2D in the two types of samples.CRC cell lines with GRIN2D overexpression or knockout were constructed to assess the effect of GRIN2D on CRC cell proliferation.Results The expression level of GRIN2D in CRC tissue was significantly higher than that in adjacent control tissue(P=0.001).The high expression of GRIN2D was associated with advanced TNM stage,deeper tumor infiltration,lymph node metastasis,and vascular cancer thrombus(x2=3.99-8.08,P＜0.05).The patients with high expression of GRIN2D had a signifi-cantly shorter disease-free survival time than those with low expression(x2=6.01,P=0.042).GRIN2D knockout could promote CRC cell apoptosis and inhibit CRC cell proliferation through the BAX/BCL-2 pathway(t=5.73-25.84,P＜0.05).Conclusion GRIN2D is highly expressed in CRC tissue,and GRIN2D knockout can inhibit CRC cell proliferation.GRIN2D is expected to be-come a new molecular therapeutic target for CRC.