共培养体系下融合魏斯氏菌XG-3胞外多糖的产量优化及特性研究OA
Optimization of Exopolysaccharide Production by Weissella confusa XG-3 in a Co-culture System and Characterization of its Properties
本研究建立融合魏斯氏菌(Weissella confusa)XG-3 与休哈塔假丝酵母(Candida shehatae)共培养体系,采用单因素法优化菌株 XG-3产胞外多糖(exopolysaccharide,EPS),并探究 EPS 的性质.结果表明,在 W.confusa XG-3单培养 4h 后接入 C.shehatae,接种比例为 1∶1,EPS 产量达到最大(56.82±0.12)g/L,是单培养的 1.63 倍.优化后 XG-3产 EPS 的最优培养条件:蔗糖 7%、酵母浸粉 0.3%、蛋白胨 1.2%、硫酸镁 0.03%、柠檬酸铵 0.4%、磷酸氢二钾 0.3%、吐温-80 0.05%、乙酸钠 5%、牛肉膏 10%、初始 pH 6.0、培养温度 30℃、摇床转速 120 rpm.优化后菌株 W.confusa XG-3的 EPS 产量为(89.32±0.55)g/L,较优化前提高 2.6 倍.共培养 XG-3 EPS 具有良好的乳化性(对豆油和汽油的乳化率分别为(92.65±0.07)%和(81.08±0.09)%)、特性粘度和重金属吸附能力.
In this study,a co-culture system integrating Weissella confusa XG-3 and Candida shehatae was established.The One-Factor-At-A-Time method was used to optimize exopolysaccharide production by XG3,and the properties of the produced EPS were investigated.The results demonstrated that when C.shehatae was inoculated 4 h after W.confusa XG-3 at an inoculation ratio of 1∶1,the EPS yield reached its maximum(56.82±0.12)g/L,which was 1.63 times higher than that obtained from monoculture.The optimal medium formulation for EPS production was composed of 7%sucrose,0.3%yeast extract,1.2%peptone,0.03%magnesium sulfate,0.4%ammonium citrate,0.3%dipotassium phosphate,0.05%Tween-80,5%sodium acetate,and 10%beef extract.The optimal culture conditions were an initial pH of 6.0,an incubation temperature of 30℃,and a shaking speed of 120 rpm.Based on optimization,the EPS yield of the W.confusa XG-3 strain reached(89.32±0.55)g/L,representing a 2.6-fold increase compared to before optimization.The EPS produced under co-culture exhibited excellent emulsifying activity(the emulsification indices for soybean oil and gasoline were(92.65±0.07)%and(81.08±0.09)%,respectively),a high intrinsic viscosity,and strong heavy-metal adsorption capacity.
曹文杰;赵守祺;高古博;杨舒涵;杜仁鹏;赵丹
黑龙江大学生命科学学院,农业微生物技术教育部工程研究中心,黑龙江省寒区植物基因与生物发酵重点实验室,黑龙江省普通高校微生物重点实验室,黑龙江 哈尔滨 150080黑龙江大学生命科学学院,农业微生物技术教育部工程研究中心,黑龙江省寒区植物基因与生物发酵重点实验室,黑龙江省普通高校微生物重点实验室,黑龙江 哈尔滨 150080黑龙江大学生命科学学院,农业微生物技术教育部工程研究中心,黑龙江省寒区植物基因与生物发酵重点实验室,黑龙江省普通高校微生物重点实验室,黑龙江 哈尔滨 150080黑龙江大学生命科学学院,农业微生物技术教育部工程研究中心,黑龙江省寒区植物基因与生物发酵重点实验室,黑龙江省普通高校微生物重点实验室,黑龙江 哈尔滨 150080黑龙江大学生命科学学院,农业微生物技术教育部工程研究中心,黑龙江省寒区植物基因与生物发酵重点实验室,黑龙江省普通高校微生物重点实验室,黑龙江 哈尔滨 150080黑龙江大学生命科学学院,农业微生物技术教育部工程研究中心,黑龙江省寒区植物基因与生物发酵重点实验室,黑龙江省普通高校微生物重点实验室,黑龙江 哈尔滨 150080
生物科学
共培养融合魏斯氏菌胞外多糖优化性质
co-cultureWeissella confusaexopolysaccharideoptimizationproperties
《南京师大学报（自然科学版）》 2026 (2)
49-58,10
国家自然科学基金项目(32072189)、黑龙江省高校基本科研业务费科研项目(2024-kYYWF-0127).
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