酒糟中原木质素和纤维素高效水解分离与回收OA

The high-value utilization of distillers' grains has gradually attracted attention with the advances of green and sustainable development in the liquor industry.In this study,an integrated strategy based on a combination of enzymatic pretreatment and alkaline hydrolysis,was developed for the full-component separation of distillers' grains and the intact recovery of native lignin and cellulose.Initially,amylase and saccharase were used stepwise to remove starch and soluble sugars from the distillers' grains,followed by alkaline hydrolysis under mild conditions to separate lignin and cellulose.The effects to the product,of hydrolysis temperature(60～120℃)and time(48～66 h)were systematically investigated.Efficient separation of lignin and cellulose was achieved under the conditions of 100℃and 0.5 mol/L NaOH for 48 h,the lignin recovery reached 91.4%.FT-IR,NMR,GPC,TGA,and XRD confirmed that the recovered lignin retained its complete structure,exhibited a moderate molecular weight with a narrow distribution(Mw 13 kDa,PDI 1.3),and the cellulose was of high purity and exhibited good crystallinity.SEM observations revealed that the lignin had a uniform spherical morphology and the cellulose fibers showed clear fibrous structures.This method provides a simple and efficient separation pathway for the high-value components of distillers' grains,demonstrating potential for resource utilization.