水龙骨粗多糖-硫辛酸-壳聚糖复合水凝胶在伤口修复中的应用OA

This study aimed to investigate the antioxidant activity of crude polysaccharides from the traditional Chinese medicine Rhizoma polypodiodis nipponicae and to evaluate the wound-healing efficacy of the Rhizoma polypodiodis nipponicae polysaccharide(RPNP)-lipoic acid(LA)-chitosan(CS)composite hydrogel in a full-thickness dorsal skin wound model in mice.RPNP was extracted via water extraction and ethanol precipitation,followed by deproteinization using the Sevage method.In vitro antioxidant capacity of RPNP was assessed using DPPH,ABTS+,and hydroxyl radical scavenging assays.CS served as a scaffold for the composite hydrogel,with LA grafted onto CS via an amide reaction.The RPNP-LA-CS composite hydrogel was prepared by incorporating RPNP,followed by self-assembly of disulfide bonds in LA under 365 nm UV irradiation.The biocompatibility of the composite hydrogel was assessed using a hemolysis assay,and its antibacterial activity against Staphylococcus aureus(S.aureus)and Pseudomonas aeruginosa(P.aeruginosa)was evaluated.A full-thickness dorsal skin wound model in mice was established to examine the wound-healing efficacy of the composite hydrogel,and histological changes were examined using hematoxylin and eosin(H&E)staining.Results demonstrated that RPNP exhibited significant scavenging activity against DPPH,ABTS+,and hydroxyl radicals,with half-maximal inhibitory concentrations(IC50)of(1.97±0.22),(1.86±0.27),and(5.48±0.48)mg/mL,respectively.The RPNP-LA-CS composite hydrogel showed excellent biocompatibility in hemolysis assays and remarkable antibacterial activity against both S.aureus and P.aeruginosa,achieving inhibition ratios exceeding 98%for both strains.In the mouse wound model,the composite hydrogel significantly promoted wound healing.Histological analysis using H&E staining revealed that skin tissues in the RPNP-LA-CS group were well-repaired,maintaining an intact structure and exhibiting superior tissue regeneration compared with the control groups.These results provide new insights into the antioxidant activity of RPNP and underscore its potential applications in trauma repair and regenerative medicine.