miR-34c-5p介导姜黄素降低HepG2脂质堆积的作用OA
miR-34c-5p Mediated Curcumin's Effect on Reducing Lipid Accumulation in HepG2 Cells
为了探讨姜黄素在转录水平上对脂质代谢的潜在调控机制,以游离脂肪酸诱导的肝癌细胞HepG2 为模型,通过转染调节细胞内 miRNA 水平,进而阐释姜黄素是否通过调控 miRNA 改善肝细胞脂质代谢紊乱.结果显示,姜黄素能上调 miR-34c-5p 表达,过表达 miR-34c-5p 后细胞中脂质沉积减少,甘油三酯与总胆固醇含量降低,脂肪酸合成基因和胆固醇合成基因表达下调,而脂肪酸分解基因和甘油三酯分解基因表达上调.进一步研究发现,在脂肪酸合成、延伸和甘油酯代谢通路中的部分基因也受 miR-34c-5p 的调控.因此,姜黄素可通过提高HepG2 中miR-34c-5p 的表达水平减少脂质堆积,从而缓解脂质代谢紊乱.
In order to explore curcumin's regulatory mechanism on lipid metabolism at the transcriptional level,free fatty acid-induced HepG2 cells were used as the model in this study,and cell transfection was em-ployed to adjust the intracellular miRNA levels to address whether curcumin ameliorated hepatic lipid metabolic disorders via miRNA modulation.The results demonstrated that curcumin upregulated the expression of miR-34c-5p.Overexpression of miR-34c-5p led to decreased lipid accumulation in cells,reduced content of triglyc-eride and total cholesterol,as well as downregulated expression of fatty acid and cholesterol synthesis genes,and upregulated expression of fatty acid and triglyceride catabolism genes.Further investigation revealed that some genes involved in fatty acid synthesis and elongation and glycerolipid metabolism pathways were regulated by miR-34c-5p.In conclusion,curcumin alleviated lipid metabolic disorders by increasing miR-34c-5p,thereby reducing lipid accumulation in HepG2 cells.
武筱雯;王浩宇;林彦祺;付凯丽;邵鹏;林静瑜;李桂玲
集美大学海洋食品与生物工程学院,福建 厦门 361021集美大学海洋食品与生物工程学院,福建 厦门 361021集美大学海洋食品与生物工程学院,福建 厦门 361021集美大学海洋食品与生物工程学院,福建 厦门 361021集美大学海洋食品与生物工程学院,福建 厦门 361021集美大学海洋食品与生物工程学院,福建 厦门 361021集美大学海洋食品与生物工程学院,福建 厦门 361021
轻工纺织
微小RNA姜黄素脂质代谢肝癌细胞HepG2转录调控
miRNAcurcuminlipid metabolismHepG2 cellstranscriptional regulation
《集美大学学报（自然科学版）》 2026 (2)
164-175,12
国家自然科学基金项目(31771972)福建省自然科学基金项目(2017J01447)
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