柠檬酸对斑节对虾生长性能、免疫抗氧化性能及肌肉营养成分的影响OA

In order to investigate the effects of dietary citric acid(CA)supplementation on growth per-formance,immune antioxidant properties and muscle nutrient composition in an African strain of black tiger shrimp(Penaeus monodon),a total of 900 shrimp(initial body weight(3.36±0.48)g)were selected,and the control group(G0)received a basal diet,while experimental groups(G1-G5)were fed diets supplemented with 0.1%,0.2%,0.3%,0.4%,and 0.5%CA for 49 days,respectively,and the effects of CA on the growth per-formance,immune antioxidant properties and muscle nutrient composition of Penaeus vannamei were studied.The results indicated:1)The G1 exhibited the highest final body weight,weight gain rate,and specific growth rate,significantly surpassing the G0(P＜0.05),along with the lowest feed conversion ratio;2)α-amylase and trypsin activities in G1 were significantly elevated compared to the G0(P＜0.05);3)Lysozyme content showed no statistical significance,G1 demonstrated the highest alkaline phosphatase,acid phosphatase and total antioxi-dant capacity(T-AOC),all exceeding the G0(P＜0.05);4)A negative correlation between superoxide dis-mutase(SOD)activity and malondialdehyde(MDA)concentration was observed across groups,with the CA groups showing superior values;5)G1 displayed the highest proportions of umami-associated amino acids and total amino acids(P＜0.05);6)Saturated fatty acids and polyunsaturated fatty acids in G1 were significantly higher than in the G0(P＜0.05).In conclusion,dietary CA supplementation at 0.1%optimally enhanced growth,antioxidant capacity and muscle nutritional quality in P.monodon,demonstrating its potential for aqua-culture applications.