饲料中添加辅酶Q10对花鲈抗高温胁迫能力的影响OA

To develop a functional feed that enhances the high-temperature stress resistance of Lateolabrax maculatus,this study focused on L.maculatus and added 0,10 and 20 mg/kg of coenzyme Q10 to the basic feed,denoted as Con,C10 and C20,respectively.In a high-temperature stress environment with a water tempera-ture of 33℃,these three types of feed were fed to sea bass separately for 56 days.The results demonstrated that adding 10 or 20 mg/kg of coenzyme Q10 to the diet could significantly increase the lower weight gain of spotted seabass under heat stress,and alleviate the morphological damage of gill and liver of spotted seabass,reduce the fatty deposition of liver,significantly increase the activities of total antioxidant capacity(T-AOC),superoxide dismutase(SOD),and catalase(CAT)of spotted seabass,and significantly decrease the malondial-dehyde(MDA)content in liver and alleviate oxidative stress.Further studies revealed that coenzyme Q10 also promoted the mitochondrial function of the spotted seabass,significantly increased the activities of citrate syn-thase(CS)and succinate dehydrogenase(SDH),increased the rate of the mitochondrial tricarboxylic acid cy-cle,and significantly up-regulated the relative expression of the mitochondrial autophagy and generation-related genes pink1,mul1,atg5,pgc-1α,pgc-1β and nrf1,which promoted the mitochondrial self-renewal and repair,and maintain normal mitochondrial function.So adding either 10 or 20 mg/kg of coenzyme Q10 to the diet could enhance the spotted seabass's ability to withstand heat stress and significantly improve its growth perform-ance.