参芪黄颗粒对脓毒症大鼠胃肠功能障碍和肠道菌群的影响OA

Objective To investigate the efficacy of Shenqihuang Granules in alleviating gastrointestinal dysfunction in sepsis model rats.Methods The cecal ligation and puncture(CLP)method was used to establish a sepsis rat model.Rats were randomly divided into the sham-operation group,the CLP group,the low dose Shenqihuang group,the medium dose Shenqihuang group,the high dose Shenqihuang group and the dexamethasone group.After modeling,animals were treated for three consecutive days.Rats in the low dose Shenqihuang group,the medium dose Shenqihuang group,the high dose Shenqihuang group were administered Shenqihuang Granules by gavage.The dexamethasone group received dexamethasone by gavage,and the remaining groups were given an equal volume of distilled water by gavage.Enzyme-linked immunosorbent assay was used to detect serum interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),anti-inflammatory factors interleukin-10(IL-10)and arginase-1(Arg-1),and intestinal injury markers diamine oxidase(DAO)and D-lactic acid(D-Lac).Hematoxylin-Eosin(HE)staining was used to observe the histopathological changes in colonic tissue.The gut microbiome is sequenced using metagenomics to analyze the compositional differences in bacterial phyla across groups and identify characteristic microbes.Results Shenqihuang Granules significantly down-regulated serum pro-inflammatory cytokines IL-6 and TNF-α(P<0.01,P<0.05)and elevated anti-inflammatory factors IL-10 and Arg-1(P<0.01,P<0.05).It also improved colon histopathological changes,down-regulated intestinal injury markers DAO and D-Lac(P<0.01),and modulated gut microbiota to restore microbial balance.Conclusion Shenqihuang Granules offer therapeutic benefits for sepsis rats by regulating inflammation,alleviating intestinal injuries,and modulating gut microbiota.