百香果AQP基因家族的鉴定及其与果皮皱缩关系OA

To investigate the role of aquaporins(AQPs)in peel shrinkage of postharvest passion fruit,this study firstly identified AQP family members from the passion fruit genome and conducted a comprehensive bioinformatics analysis.Subsequently,the shrinkage index,weight loss rate,peel moisture content,and expression levels of PeAQP genes were monitored during room-temperature storage.A total of 39 PeAQP genes were identified and classified into four subfamilies,with deduced amino acid lengths ranged from 80 to 848 residues,molecular weights of 8.7 to 94.1 kDa,and isoelectric points(pI)ranging from 4.9 to 9.97.Moisture dynamics analysis revealed that with increasing shrinkage index,both total water content and free water content in the peel declined,whereas bound water and semi-bound water content firstly increased and then decreased.A significant positive correlation was observed between free water content and total water content(P<0.05),suggesting that free water loss is the primary driver of peel shrinkage.Gene expression analysis showed that PeTIP2-2,PeNIP1-1,PeNIP5,PeTIP5-1,PePIP2-8,PeSIP2-1,PeSIP1-1,PePIP2-5,PePIP3-1,PePIP3-2,PeTIP2-1,PeTIP4-1,PePIP1-6,PeNIP2-2,and PeNIP4 exhibited upregulated expression during shrinkage.Notably,the expression levels of PeNIP4,PeNIP2-2,PePIP1-6,and PePIP3-2 exhibited a significantly positive correlation with the peel shrinkage index(P<0.05).These findings provide a theoretical foundation for further elucidation of the role and regulatory mechanisms of PeAQP proteins in postharvest passion fruit peel shrinkage.