藿香叶绿绒蒿MYB家族鉴定及与花色相关基因分析OA

For the identification and analysis of the MYB transcription factor family in blue alpine flowers Meconopsis betonicifolia,full-length transcriptome sequencing data and bioinformatics method was used,the members of M.betonicifolia MYB transcription factor family were identified,and their physicochemical properties,phylogenetic evolution,conservative motif and domain were analyzed.Using RT-PCR technology,MbMYB22 gene related to flower color was cloned from the petals of M.betonicifolia,and the expression of MbMYB22 gene in various tissues of M.betonicifolia was verified through qRT-PCR technology.The relationship between the total flavonoids content in different tissues and the total amount of cyanidin in petals at different periods and the expression of MbMYB22 gene were analyzed to verify its role in flower color regulation.A total of 69 MbMYB transcription factors were identified in the full-length transcriptome of M.betonicifolia,including 32 1R-MYB,34 R2R3-MYB and 3 3R-MYB transcription factors.The molecular masses of the deduced proteins ranged from 14 467.7 to 20 509 595.1 Da,and all identified proteins were hydrophilic.Phylogenetic analysis showed that the R2R3-MYB transcription factors from M.betonicifolia and A.thaliana were grouped into 35 subgroups in the combined phylogenetic tree,seven of which contained M.betonicifolia members and corresponded to previously classified subgroups in A.thaliana.Among them,MbMYB22 clustered with the flower color-related S6 subgroup and showed the closest phylogenetic relationship to PsMYB.The expression pattern of MbMYB22 is significantly positively correlated with the accumulation pattern of both total flavonoids content and total cyanidins content.The cloning of MbMYB22 gene,qRT-PCR analysis,and quantification of total flavonoids in different tissues and cyanidin in petals at different periods were carried out suggested the involvement of the MbMYB22 gene in flower color regulation.This study provides a theoretical basis for further investigations into the regulatory mechanisms of flower coloration regulated by the MYB transcription factor family in M.betonicifolia.