黄伞航天诱变菌株变异稳定性、农艺学性状及营养成分评价OA

Pholiota adiposa is a high-value edible fungus,but its existing strains still face challenges in industrial applications,such as low first flush efficiency and long fruiting time.Space mutation breeding has many advantages,including high mutation frequency,diverse mutation types,and the generation of variations that are rarely attainable through conventional breeding methods.To explore the potential of aerospace mutagenesis technology in edible fungi breeding and obtain high-quality germplasm resources,in this study,the HS5 strain of P.adiposa was used as the starting material and exposed to mutagenesis during its flight aboard the Shenzhou XII manned spacecraft.After the spacecraft returned,50 mutant strains were obtained by separation and purification.Through antagonistic tests,subculture,nutritional content determination,and fruiting evaluations,the differences between the space-mutated strains and the control strain,as well as the stability of the space mutants were systematically analyzed.The results indicated that the growth rate of P.adiposa strains significantly increased after space mutagenesis,and stable variants could be obtained following continuous subculturing.Based on the analysis of nutritional content and agronomic traits of P.adiposa,significant differences were observed in total saccharide,proteins,dietary fibers content,as well as fruiting bodies agronomic traits between stable mutant strains and control strains.Selected strains exhibiting desirable characteristics include:HT6,a space mutation strain with a biological efficiency of 81.80%during the first flush,a longer stipe length,and suitability for industrial production;HT15,which has a significantly higher total saccharide content compared to the control strain;HT37,with significantly higher protein and total saccharide contents;and HT1,which exhibits significantly higher total saccharide and dietary fiber contents than the control strain.This study demonstrates that space mutagenesis technology can effectively enrich P.adiposa germplasm resources,facilitate the acquisition of novel germplasm,and provide a valuable reference for breeding new varieties of edible fungi.