山奈酚通过激活Wnt/β-catenin通路抑制神经干细胞凋亡改善脑梗死预后的研究OA

Objective To investigate the effect of kaempferol on inhibiting apoptosis of neural stem cells by activating the Wnt/β-catenin pathway to improve the prognosis of cerebral infarction.Methods In vivo,kaempferol was administered to middle cerebral artery occlusion(MCAO)mouse model to determine the effects of kaempferol on infarct volume and nerve function,and the changes of Wnt/β-catenin pathway related proteins in each group were compared by western blot analysis to understand the mechanism of action.OGD/reoxidation(R)models of neural stem cells(NSCs)were established,and the effects of kaempferol on cell viability,migration and apoptosis were evaluated by cell counting kit 8,Transwell assay,flow cytometry and TUNEL analysis.Results Kaempferol improved nerve function defect,reduced infarct volume and promoted nerve regeneration in mice with cerebral infarction.Secondly,kaempferol can also promote the cell viability and migration of OGD/R treated NSCs and inhibit cell apoptosis.In addition,kaempferol enhances the activity of the Wnt/β-catenin pathway,and blocking the Wnt/β-catenin pathway can cause the loss of biological function of kaempferol-mediated NSCs.Conclusion Kaempferol can inhibit cerebral infarction by promoting NSCs activity,migration and inhibiting apoptosis.Kaempferol can improve nerve function injury after cerebral infarction by activating Wnt/β-catenin pathway,which is expected to be a new drug for the treatment of ischemic cerebral infarction.