RBM15B在阿尔茨海默病中的表达变化及其对神经元损伤的影响OA

Objective To investigate the expression of RNA binding motif protein 15B(RBM15B)in Alzheimer's disease(AD)and its effect on neuronal injury.Methods The expression of RBM15 B in different brain regions of AD patients was analyzed by AlzData database.The AD model of PC-12 cells induced by Aβ25-35 was established to detect the effect of different concentrations of Aβ 25-35 on the expression of RBM15B.The expression level of RBM15B was regulated by siRNA knockdown and overexpression vector transfection,and the effects on cell viability and apoptosis were observed.APP/PS1 transgenic AD mouse model was established for behavioral detection and Western blot analysis.Results Bioinformatics analysis showed that RBM15B was significantly up-regulated in the frontal cortex and entorhinal cortex of AD patients.With the increase of A β 25-35 concentration,the expression of RBM15B in PC-12 cells increased in a concentration-dependent manner,while the cell viability decreased and the apoptosis increased.RBM15B knockdown significantly improved Aβ25-35-induced cell damage,while overexpression significantly aggravated cell damage.The expression of RBM15B protein in the brain tissue of AD mice was significantly increased,and showed significant learning and memory impairment and decreased new object recognition ability.Conclusion RBM15B is significantly up-regulated in AD patients and AD models,and its high expression can aggravate neuronal damage.Knockdown of RBM15B expression has a protective effect on neurons,suggesting that RBM15B may be a potential target for AD treatment.