脉冲电场强化厌氧氨氧化颗粒污泥脱氮性能及微生物菌群结构响应OA
Enhancing nitrogen removal of anammox granular sludge with pulsed electric field and the response of microbial community structure
本研究针对厌氧氨氧化菌生长缓慢并在高氮负荷下易受基质抑制、菌群活性降低等问题,引入环形脉冲电场(1.5 V电压,4 h通电时长)调控策略,系统探究其对颗粒污泥脱氮性能及微生物群落的优化机制.通过对比 2组厌氧序批式反应器(R1无电场、R2施加电场)在不同进水氮负荷下的运行效果发现:当氮负荷低于 1 155 mg·(L·d)-1时,R2总氮去除率较 R1提升了 7.5%～17.0%,生物量、比厌氧氨氧化活性和胞外聚合物分泌量分别增加了 5%～7%、21%～71%和 54%～77%,表明外加电场通过促进功能菌的代谢活性与胞外聚合物的分泌,强化厌氧氨氧化作用;但当氮负荷大于 1 320 mg·(L·d)-1 时,NO2--N毒性效应主导,外加电场对厌氧氨氧化作用的强化效果减弱,甚至转为抑制.微生物群落分析结果表明,中低负荷下 R2的 Planctomycetes门及厌氧氨氧化菌(Candidatus Brocadia和Candidatus Jettenia)相对丰度较 R1提高,菌群丰富度(Chao1指数)和多样性(Shannon/Simpson指数)同步增强,而在高负荷时 R2菌群丰度反低于 R1.主成分分析和冗余分析进一步揭示,中低负荷下脉冲电场是驱动菌群差异的关键因子,而高负荷时 NO2--N质量浓度成为主导调控参数.本研究为提升厌氧氨氧化工艺抗冲击负荷能力及工程化应用提供理论参考.
This study addresses the challenges of slow growth of anaerobic ammonium oxidation(anammox)bacteria,their susceptibility to inhibition under high nitrogen loads,and reduced microbial activity.A circular pulsed electric field(1.5 V voltage,4 h electrification duration)was introduced as a regulatory strategy to systematically explore its optimization mechanisms on the nitrogen removal performance of anammox granular sludge and the associated microbial communities.By comparing the operational effects of two anaerobic sequencing batch reactors(R1:no electric field;R2:electric field applied)under varying influent nitrogen loads,the results revealed that when at loads below 1 155 mg·(L·d)-1,the total nitrogen removal efficiency of R2 was 7.5%～17.0%higher than that of R1.The biomass,specific anammox activity,and extracellular polymeric substance(EPS)secretion in R2 were also enhanced by 5%～7%,21%～71%,and 54%～77%,respectively,indicating that the external electric field strengthened the system by promoting metabolism of functional bacteria and EPS secretion.However,when the nitrogen load exceeded 1 320 mg·(L·d)-1,inhibition from NO2--N toxicity became dominant,and the enhancing effect of electric field weakened or even reversed.Microbial community analysis demonstrated that under moderate-to-low loads,the relative abundances of Planctomycetes phylum and anammox bacteria(Candidatus Brocadia and Candidatus Jettenia)in R2 were higher than those in R1,alongside elevated microbial richness(Chao1 index)and diversity(Shannon/Simpson indices).Conversely,under high nitrogen loads,microbial richness in R2 decreased below that of R1.Principal component analysis(PCA)and redundancy analysis(RDA)further confirmed that the pulsed electric field was the key driver of microbial community divergence under moderate-to-low loads,while the NO2--N concentration became the dominant regulatory parameter under high loads.This study provides theoretical insights for enhancing the shock-load resistance and promoting the engineering application of the anammox process
王旭中;张宁;张鹏宇;苗娟;张瑞昌;周鸣;魏学锋
河南科技大学化学化工学院,洛阳 471000河南科技大学化学化工学院,洛阳 471000河南科技大学化学化工学院,洛阳 471000河南科技大学化学化工学院,洛阳 471000河南科技大学化学化工学院,洛阳 471000河南科技大学化学化工学院,洛阳 471000河南科技大学化学化工学院,洛阳 471000
资源环境
脉冲电场颗粒污泥厌氧氨氧化微生物群落
pulsed electric fieldgranular sludgeanammoxmicrobial community
《环境工程学报》 2026 (3)
752-762,11
河南省科技攻关项目(242102321064)
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