枳实水提物的降脂活性OA

To investigate the lipid-lowering effects and oxidative stress modulation mechanisms of Aurantii Fructus Immaturus water extract(AFIWE)on a cellular model of non-alcoholic fatty liver disease(NAFLD),this study obtained AFIWE through water reflux extraction.The cytotoxicity of AFIWE on HepG2 cells was assessed using the CCK-8 assay.For the lipid-lowering experiment,an NAFLD model was established by free fatty acid(FFA)induction,with experimental groups including a blank control group,model group,AFIWE intervention groups(25,50 and 100 µg/mL),and a positive control group(25 µg/mL silymarin).Intracellular triglyceride(TG)and total cholesterol(TC)levels were measured using commercial assay kits.Lipid droplet accumulation was observed via Oil Red O staining,and the activities of superoxide dismutase(SOD),catalase(CAT),glutathione(GSH),as well as the levels of malondialdehyde(MDA)and reactive oxygen species(ROS),were determined.Results demonstrated that AFIWE at concentrations ranging from 25 to 400 µg/mL exhibited no significant cytotoxicity(cell viability>94%).Compared with the model group,AFIWE at 50 and 100 µg/mL reduced intracellular TG content by 28.6%and 41.2%,and TC content by 25.3%and 38.7%,respectively.Lipid droplet accumulation was also reduced in a concentration-dependent manner,with the 100 µg/mL group showing lipid-lowering effects comparable to those of silymarin(p>0.05).Furthermore,AFIWE at 50 and 100 µg/mL significantly enhanced SOD activity by 21.5%and 32.8%,increased CAT activity by 18.7%and 29.4%,elevated GSH content by 23.1%and 35.6%,reduced MDA levels by 26.8%and 40.3%,and decreased ROS levels by 30.2%and 42.5%,respectively.In conclusion,AFIWE exerted lipid-lowering effects by regulating oxidative stress and lipid metabolism,providing experimental evidence for the prevention and treatment of NAFLD.