丹酚酸B对糖尿病合并慢性牙周炎及PTEN/Nrf2信号通路的影响OA

Objective:To investigate the therapeutic effect of salvianolic Acid B(salB)and its impact on the PTEN/Nrf2 signaling pathway in a rat model of DMCP.Methods:A total of 38 male SD rats were ran-domly assigned to control group(CON,n=8)and DMCP model group(DMCP,n=30).The DMCP model was established by inducing DM with streptozotocin and combining it with maxillary ligation to induce CP.The 25 successfully modeled rats were then randomly divided into:DMCP group(n=9),salB treatment group(n=8),and positive control group(metformin,MET,n=8).Each group was administered the corresponding drug or an equal volume of normal saline via gastric intubation daily for 6 weeks.In vivo ROS levels were as-sessed using live fluorescence imaging.Alveolar bone defect severity was quantified on micro-CT images by measuring the linear distance between the CEJ and the ABC.Histological analysis was performed using H&E and Masson staining.Levels of pro-inflammatory cytokines(TNF-α,IL-1β,IL-6,IL-17)in the gingival tissue surrounding the ligature site were measured using ELISA kits.The impact of macrophage polarization on periodontitis development was detected using immunofluorescence staining.Expression levels of PTEN and Nrf2 were determined by RT-PCR and Western blot.Results:Compared to the CON group,the DMCP group showed a statistically significant increase in the CEJ-ABC distance(P＜0.05).Histological analysis revealed severe alveolar bone damage accompanied by an inflammatory response in the DMCP group.ROS and pro-in-flammatory cytokine(TNF-α,IL-1β,IL-6,IL-17)levels were significantly elevated in the DMCP group(P＜0.05).RT-PCR and Western blot results indicated a statistically significant decrease in PTEN expres-sion and an increase in Nrf2 expression in the DMCP group(P＜0.05).Compared to the DMCP group,the salB-M and salB-H groups exhibited a statistically significant reduction in the CEJ-ABC distance,restoration of alveolar bone damage,and significantly lower levels of ROS and pro-inflammatory cytokines(P＜0.05).After salB treatment,PTEN expression was upregulated and Nrf2 expression was further upregulated,with sta-tistical significance(P＜0.05).Conclusion:SalB alleviated the destructive effects of DMCP on alveolar bone,reduced ROS levels,modulated macrophage polarization and inflammatory cytokine levels,which might be related to its regulation of PTEN/Nrf2 pathway.