具有聚集诱导发光性质的高灵敏度和高光稳定性的脂滴荧光探针OA

Two novel aggregation-induced emission(AIE)-active probes,TPA-H and TPA-2F,were designed and synthesized based on a triphenylamine(TPA)core.Systematic characterization demonstrated that both probes exhibit excellent biocompatibility(cell viability>90%at concentrations up to 50 µmol/L)and outstanding LD-targeting speci-ficity with minimal colocalization with other organelles such as mitochondria and lysosomes.During early differentia-tion of 3T3-L1 adipocytes,both TPA-2F and TPA-H clearly visualized small and nascent LDs that were difficult to be detected with BODIPY,indicating superior imaging sensitivity compared to the existing fluorescent probes for LDs.Moreover,TPA-2F demonstrated exceptional photostability,retaining over 90%of its initial fluorescence intensity after 100 continuous laser scanning cycles,significantly outperforming TPA-H.This work not only provides two high-performance LD imaging tools but also highlights the potential of AIE luminogens(AIEgens)in organelle-specific bioimaging,offering promising avenues for early diagnosis and mechanistic research of lipid-related metabolic diseases.