基于SRAP分子标记分析贵州芝麻种质资源的遗传多样性OA

[Objective]The genetic diversity of sesame germplasm resources was explored to provide a reference basis for preservation and utilization of sesame germplasm resources in Guizhou.[Method]The leaves of 70 sesame germplasm resources from Anshun,Bijie,Southeast Guizhou,South Guizhou,Tongren and Zunyi in Guizhou were amplified by PCR and using SRAP primers,and the genetic parameters of polymorphic loci,Nei's gene diversity index(H)and Shannon information index were analyzed.[Result]A total of 232 loci were detected in 70 sesame germplasm resources by using 16 pairs of SRAP primer combinations,and the polymorphic loci were 116.The Nei's gene diversity index(H)and Shannon information index(I)of 70 sesame germplasm resources was 0.288 1 and 0.448 2 respectively.The genetic similarity coefficient ranged from 0.422 4 to 0.827 6 with an average value of 0.636 4.70 sesame germplasm resources from Guizhou were divided into 6 groups according to the geographical origin.The Nei's gene diversity index(H),Shannon information index(I),genetic consistency and genetic distance were 0.160 7-0.296 6,0.234 6-0.449 7,0.857 5-0.998 0 and 0.002 0-0.153 7 respectively.70 sesame germplasm resources were divided into 5 groups when the genetic similarity coefficient was 0.63 based on UPGMA genetic clustering[Conclusion]The genetic diversity of sesame germplasm resources is relatively abundance in Guizhou.The genetic consistency of sesame germplasm resources in Zunyi and South Guizhou is the highest,but the genetic consistency of sesame germplasm resources in Tongren and Anshun is the lowest.The clustering results of genetic relationships have no necessary connection with geographical origin.