基于Fas信号通路探讨荣筋拈痛方改善骨关节炎软骨退变的作用机制OA

Objective:To investigate the mechanism of Rongjin Niantong formula in improving cartilage degeneration in osteo-arthritis(OA)based on the Fas signaling pathway.Methods:Twenty-four male SD rats were divided into a control group of 8 rats and a modeling group of 16 rats.The OA rat model was established using the modified Hulth method in the modeling group,while a 1 cm incision was made on the skin of the medial side of the right knee joint in the control group,which was sutured immediately after incision.The successfully modeled rats were randomly divided into model group and Rongjin Niantong formula group,with 8 rats in each group.The Rongjin Niantong formula group was administered with Rongjin Niantong formula solution by gavage at a dosage of 4.6 g/(kg·d),while the control and model groups were administered with physiological saline by gavage at a dosage of 4 mL/(kg·d).Gavage was performed once daily,and the treatment was continued for 12 consecutive weeks.The appearance and daily behavior of the three groups of rats were observed,and their body weight was measured at a fixed time each week during the experi-mental period.The serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL),total protein(TP),globulin(GLB),creatinine(CR),blood urea nitrogen(BUN),and uric acid(UA)were measured in three groups.HE staining was used to observe the pathological morphological changes of cartilage tissue.Western blot was used to detect the protein expressions of Fas,Fas-associated death domain protein(FADD),p-FADD and p53 in cartilage tissue.Results:The overall physio-logical state of the rats was good,with normal food and water intake,and no abnormal changes in behavioral activities were observed during the experimental period.HE staining revealed that the cartilage structure in the control group was intact;the structure of each layer of cartilage in the model group was significantly damaged,while the layers of cartilage in the Rongjin Niantong formula group were relatively clear.There were no statistically significant differences in body weight and post-intervention serum levels of ALT,AST,TBIL,TP,GLB,CR,BUN,and UA among the three groups with the same intervention duration(P>0.05).Compared with the control group,the protein expressions of Fas,p53,and FADD in the cartilage tissue of the model group significantly increased(P<0.05),while the protein expression of p-FADD and the ratio of p-FADD/FADD significantly decreased(P<0.05).Compared with the model group,the protein expressions of Fas,p53,and FADD in the Rongjin Niantong formula group significantly decreased(P<0.05),while the protein expression of p-FADD and the ratio of p-FADD/FADD significantly increased(P<0.05).Conclusion:Rongjin Niantong Formula can inhibit chondrocyte apoptosis and improve cartilage degeneration in OA rats by regulating the Fas sig-naling pathway.