预知子提取物的不同分离部位对脂多糖诱导BV2细胞介导神经炎症作用及其机制的研究OA

Objective To investigate the anti-neuroinflammatory effects of different fractions of the Akebiae Fructus extract on lipopoly-saccharide(LPS)-induced BV2 cells and to explore the underlying mechanisms.Methods The Akebiae Fructus extract(JE)was ob-tained by ethanol reflux extraction,and the systematic solvent extraction method was used to separate the n-hexane fraction(JE1),di-chloromethane fraction(JE2),ethyl acetate fraction(JE3),and n-butanol fraction(JE4).An inflammatory model was established using LPS-induced BV2 cells;MTT assay was used to observe the cell viability.The content of NO in cell supernatant was measured by Griess reagent.The levels of PGE2,IL-6 and TNF-α were detected by ELISA kits.Detection of COX-2、iNOS protein and MAPK、NF-κB sig-naling pathway related protein expression levels using Western Blot method.Results Both JE1 and JE2 significantly reduced the levels of NO,PGE2,IL-6,TNF-α and the protein expressions of COX-2 and iNOS in LPS-induced BV2 cells,and also significantly decreased the protein expressions of P38,ERK,JNK,and NF-κB.Conclusion The JE1 and JE2 fractions of the Akebiae Fructus extract can effec-tively prevent LPS-induced neuroinflammatory responses occur in BV2 cells,and the underlying mechanism is associated with the inhibi-tion of the inflammatory NF-κB and MAPK signaling pathways.