前处理和胚切割方式对小黑麦成熟胚愈伤组织培养的影响OA

Mature triticale embryos were used as explants to investigate the effects of different disinfection,embryo-cutting and seed-soaking methods on callus induction and sprout growth.Various concentrations of ethanol and sodium hypochlorite solution were used for different durations for disinfecting triticale seeds.The callus induction frequency was higher and the rate of bacterial contamination was lower than those in the control when applying 75%ethanol for 1 min and 20%sodium hypochlorite solution for 15 min for disinfecting triticale callus,which was the most effective pretreatment disinfection method.Soaking seeds with 1 g·L-1 2,4-D or picloram solution did not increase the frequency of callus induction but markedly reduced the sprout rate.Transecting two-thirds of the embryos considerably increased the callus induction frequency.Combining this transection with soaking the seeds in 1 g·L-1 2,4-D was found to be an effective preinduction treatment.These results provide technical support for tissue culture and developing triticale germplasm resources.