植物源复合不育剂ND-2的配比及其对昆明小鼠的不育效果OA

The sterile control technique is considered a potential tool for preventing and managing grassland rodent pests,and it holds great significance for achieving sustainable control.In this study,seven medicinal plants with potential antifertility effects were screened out by reviewing ancient Chinese medicinal texts,and sterility control experiments were carried out using Kunming mice as research subjects.The results showed that ricinine and paeoniflorin exhibited strong antifertility effects on female and male Kunming mice,respectively.The sterility agent ND-2(Nongda-2),developed through gradient screening and formulation optimization,demonstrated significant sterility effects in both male and female Kunming mice.Compared with the control group(gavaged with 0.2 mL of edible oil),the female treatment group showed a significant increase in uterine weight due to swelling(P<0.05),an earlier onset of reproductive initiation by 7 days(P<0.05),and a 5.9%reduction in pup survival rate(P<0.05).In the male treatment group,sperm density and litter size were significantly lower than those of the control group(P<0.05);litter size was reduced by 6.8,and reproductive initiation was delayed by 5 days(P<0.05).Across both sexes,litter size was reduced by 7.7(P<0.05),pup survival decreased by 7.5%(P<0.05),and breeding initiation was delayed by 4 days compared with the control group(P<0.05).Hormone assays indicated that,after ND-2 treatment,levels of follicle-stimulating hormone(FSH),luteinizing hormone(LH),and testosterone(T)in male rats were significantly lower than those in the control group(P<0.05),while corticosterone levels were significantly higher(P<0.05).In females,all hormone levels measured were significantly higher than those of the control group(P<0.05).Histological analysis showed that treated males had reduced testicular mesenchymal cells and limited formation of mature spermatozoa,while treated females exhibited an increased number of ovarian corpora lutea along with deformation and death of endometrial epithelial cells.These findings indicate that the plant-derived compound ND-2 has strong sterility effects in both male and female Kunming mice,offering a new option for the control of the growth of grassland rat infestation populations.