基于网络药理学探讨山芋丸治疗肌少症的作用机制OA

Objective:To explore the mechanism of Shanyu Pills in the treatment of sarcopenia based on network pharmacology.Methods:The active components and corresponding targets of Shanyu Pills were screened from the TCMSP and BATMAN-TCM databases.Sarcopenia-related targets were collected from the GeneCards,DisGeNET,OMIM,and CTD databases.The overlapping targets between Shanyu Pills and sarcopenia were obtained using a Venn diagram tool.A protein-protein interaction(PPI)network of the overlapping targets was constructed using the STRING database,and the CytoHubba plugin in Cytoscape 3.10.0 was used to screen the top 10 genes with the highest MCC scores as key therapeutic targets.Gene Ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed on the overlapping targets using the Metascape database.Finally,a visual"Shanyu Pills-Active Components-Targets-Sarcopenia"network was constructed using Cytoscape 3.10.0,and the top 10 active components of Shanyu Pills were screened according to node degree.Results:A total of 156 active components were obtained from Shanyu Pills,corresponding to 1742 potential targets;2534 sarcopenia-related targets and 121 overlapping targets were identified.Among the top 10 active components,adenosine triphosphate,stigmasterol,palmitoleic acid,dopamine,and kaempferol showed positive effects on skeletal muscle.The core targets included AKT1,IL6,TNF,IGF1,and HIF1A.GO enrichment analysis identified 310 biological processes(BP),91 cellular components(CC),and 108 molecular functions(MF).The BP terms were mainly enriched in the regulation of small molecule metabolic processes,response to hormones,response to extracellular stimuli,and regulation of oxidative stress.The CC terms were mainly enriched in membrane raft,vesicle lumen,cell cortex,euchromatin,brush border,and nucleotide-activated protein kinase complex.The MF terms were mainly enriched in signal transduction receptor activity regulation,protein homodimerization activity,transcription factor binding,steroid binding,and vitamin D binding.KEGG enrichment identified 160 significantly enriched pathways,mainly including the longevity regulating pathway,arginine and proline metabolism,and calcium signaling pathway.Conclusion:Shanyu Pills may exert therapeutic effects on sarcopenia by regulating skeletal muscle protein metabolism and mitochondrial function through multiple targets and pathways.