酶菌复配和青贮天数对五节芒青贮品质的影响OA

To investigate the effects of cellulase combined with different fermentation types of lactic acid bacteria and fermentation days on the silage quality of Miscanthus floridulus,and to screen the optimal enzyme-bacteria combination and fermentation period,this experiment used M.floridulus at the flowering stage as raw material for silage.Five treatments were set up:cellulase(EX),cellulase+Lactobacillus acidophilus(EX+LA),cellulase+Lactobacillus buchneri(EX+LB),cellulase+Lactobacillus plantarum(EX+LP),and a distilled water control group(CK).The fermentation quality and nutritional quality of each treatment were determined after 30 and 50 days of ensiling,respectively.The grey correlation analysis method was used for the comprehensive evaluation of the silage quality.The results showed that the pH and the ratio of ammoniacal nitrogen to total nitrogen in all enzyme microbial treatment groups were significantly lower than those in the CK group.The EX+LP group had the highest lactic acid content(30 d:1.71 mg/mL;50 d:1.66 mg/mL),while the EX+LB group had the highest acetic acid content(30 d:0.84 mg/mL;50 d:1.03 mg/mL).The neutral detergent fiber content in all additive treatment groups was significantly lower than that in the CK group,and the EX group had the highest crude protein content(30 d:72.0 g/kg;50 d:72.8 g/kg).After 30 days of ensiling,the EX treatment was identified as the optimal scheme(correlation degree 0.990);the EX+LB group showed the poorest comprehensive performance due to its high acid detergent lignin content and low lactic acid content.Comprehensive analysis indicates that after 30 days of ensiling,treatments with cellulase alone or its combination with L.acidophilus can improve the nutritional quality during the fermentation period,while its combination with L.plantarum can optimize the fermentation quality.