枯草芽孢杆菌C08可湿性粉剂研制及其对番茄早疫病防治效果OA
Development of a Wettable Powder Formulation of Bacillus subtilis C08 and Its Biocontrol Efficacy Against Tomato Early Blight
番茄(Solanum lycopersicum)早疫病由茄链格孢菌(Alternaria solani)侵染引发,由于其潜育期短且再侵染迅速,已成为影响番茄产量与品质的重要病害之一.相比传统化学防治方法,生物防治具有环境友好和绿色可持续的优势,展现出巨大的发展潜力.本研究从健康番茄根际土壤中筛选出1株对早疫病具有显著拮抗活性的芽孢杆菌(Bacillus)(编号C08),并优化其发酵条件,以发酵产物为基础制备可湿性粉剂.结果表明:该粉剂不仅对番茄早疫病表现出较高的防治效果,还能够显著促进植株生长,提高超氧化物歧化酶(SOD)、过氧化物酶(POD)等抗氧化酶的活性,同时改善土壤中蔗糖酶与脲酶的活性,为番茄早疫病的绿色防控提供潜在的生物防治资源与应用基础.
Tomato early blight,caused by Alternaria solani,represents a significant disease that severely impacts both the yield and quality of tomatoes,primarily due to its short latent period and rapid reinfection cycle.In contrast to conventional chemical control methods,biological control offers considerable potential due to its environmentally friendly and sustainable attributes.In this study,a strain of Bacillus subtilis,designated as C08,demonstrating strong antagonistic activity against A.solani,was isolated from the rhizosphere soil of healthy tomato plants.The fermentation conditions were optimized,and a wettable powder formulation was subsequently developed using the fermentation broth as the active ingredient.The results indicated that the formulation achieved high efficacy in controlling tomato early blight,significantly promoted plant growth,enhanced the activities of antioxidant enzymes such as superoxide dismutase(SOD)and peroxidase(POD),and improved soil enzyme activities such as sucrase and urease.These findings suggest that strain C08 holds substantial promise as a biocontrol agent for the sustainable management of tomato early blight.
许韫鑫;丛煜嫣;张瑶;王傲雪
东北农业大学生命科学学院,哈尔滨 150030东北农业大学园艺园林学院,哈尔滨 150030东北农业大学生命科学学院,哈尔滨 150030东北农业大学园艺园林学院,哈尔滨 150030
农业科技
枯草芽孢杆菌番茄早疫病可湿性粉剂生物防治
Bacillus subtilistomato early blightwettable powderbiological control
《植物研究》 2026 (2)
294-309,16
国家自然科学基金创新研究群体项目(32072588)国家自然科学基金联合基金项目(U22A20495)山东省重点研发计划项目(2024LZGCQY015).
评论