复合诱导子和光质诱导子对箭叶淫羊藿悬浮细胞次生代谢产物的影响OA
Effects of Composite Inducers and Light Quality Inducers on Secondary Metabolites in Epimedium sagittatum Suspension Cells
通过添加不同浓度复合诱导子及不同光周期的光质诱导子,探讨复合诱导子和光质诱导子对箭叶淫羊藿(Epimedium sagittatum)悬浮细胞主要次生代谢产物的影响.该研究在最佳培养条件下,设计正交试验,将25、50、75 μmol·L-1茉莉酸甲酯(methyl jasmonate,MeJA),25、50、75 μmol·L-1水杨酸(salicylic acid,SA),0、10、20 mg·L-1壳聚糖(chitosan,CTS)3种诱导子进行复合诱导,并选取不同光质和光周期进行诱导.结果表明:与对照组相比,经过复合诱导子和光质诱导子处理的箭叶淫羊藿悬浮细胞中,黄酮醇苷、总黄酮、总酚酸及总皂苷等次生代谢产物含量均呈现上升趋势.F4处理组(50 μmol·L-1 MeJA、25 μmol·L-1 SA、20 mg·L-1 CTS)和G6处理组(蓝光,16 h)黄酮醇苷含量显著高于对照组,分别是对照组的2.92倍和3.79倍;F3处理组(25 μmol·L-1 MeJA、50 μmol·L-1 SA、10 mg·L-1 CTS)和G6处理组(蓝光,16 h)总黄酮含量显著高于对照组,分别为对照组的1.42倍和1.27倍;F2处理组(25 μmol·L-1 MeJA、25 μmol·L-1 SA)和G4处理组(蓝光,12 h)总酚酸含量显著高于对照组,分别为对照组的4.11倍和2.94倍;F8处理组(75 μmol·L-1 MeJA、75 μmol·L-1 SA)和G2处理组(白光,12 h)总生物碱含量高于对照组,分别是对照组的1.28倍和1.02倍;F5处理组(50 μmol·L-1 MeJA、50 μmol·L-1 SA)和G6处理组(蓝光,16 h)总皂苷含量显著高于对照组,分别是对照组的3.73倍和3.05倍.因此,添加外源诱导子是提高箭叶淫羊藿次生代谢产物的有效途径,对黄酮类化合物和总皂苷等次生代谢产物的合成与积累具有显著促进作用.
This study investigated the effects of composite inducers at varying concentrations and photomorphogenic inducers under different photoperiods on the major secondary metabolites in Epimedium sagittatum(Sieb.et Zucc.)Maxim.suspension cells.Orthogonal experiments were conducted under optimal culture conditions to evaluate the effects of three composite inducers:MeJA at 25,50,and 75 μmol·L-1;SA at 25,50,and 75 μmol·L-1;and CTS at 0,10,and 20 mg·L-1.Additionally,induction was performed under varying light qualities and photoperiods.The findings demonstrated that the levels of secondary metabolites,including flavonol glycosides,total flavonoids,total phenolic acids,and total saponins,increased in E.sagittatum suspension cells treated with composite inducers and photomorphogenic inducers compared to the control group.The F4 treatment group(50 μmol·L-1 MeJA,25 μmol·L-1 SA,20 mg·L-1 CTS)and G6 treatment group(blue light,16 h)exhibited significantly higher flavonol glycoside content,being 2.92 and 3.79-fold higher than the control,respectively.The F3 treatment group(25 μmol·L-1 MeJA,50 μmol·L-1 SA,10 mg·L-1 CTS)and G6 treatment group(blue light,16 h)demonstrated significantly higher total flavonoid content,approximately 1.42 and 1.27-fold higher than the control,respectively.The F2 treatment group(25 μmol·L-1 MeJA,25 μmol·L-1 SA)and G4 treatment group(blue light,12 h)exhibited significantly elevated total phenolic acid content,being 4.11 and 2.94-fold higher than the control,respectively.The F8 treatment group(75 μmol·L-1 MeJA,75 μmol·L-1 SA)and G2 treatment group(white light,12 h)exhibited higher total alkaloid content,being 1.28 and 1.02-fold higher than the control,respectively.The total saponin content in the F5 treatment group(50 μmol·L-1 MeJA,50 μmol·L-1 SA)and G6 treatment group(blue light,16 h)was significantly higher,being 3.73 and 3.05-fold higher than the control,respectively.These results indicated that the application of exogenous inducers effectively enhanced the synthesis and accumulation of secondary metabolites,such as flavonoids and total saponins,in E.sagittatum.
赵梦瑶;纪宝玉;李诺;韩耀祖;裴莉昕
河南中医药大学药学院,郑州 450046||重庆市药物种植研究所,南川 408435河南中医药大学药学院,郑州 450046||豫药全产业链研发河南省协同创新中心,郑州 450046||道地药材品质保障与资源持续利用全国重点实验室,北京 100700河南中医药大学药学院,郑州 450046河南中医药大学药学院,郑州 450046河南中医药大学药学院,郑州 450046
农业科技
箭叶淫羊藿悬浮细胞复合诱导子光质诱导子次生代谢产物
Epimedium sagittatumsuspension cellscomposite inducerslight quality inducerssecondary metabolites
《植物研究》 2026 (2)
280-293,14
中央本级重大增减支项目(2060302)重庆市药物种植研究所2024年刘正宇全国名老中医药专家传承工作室开放课题(2024jbky-kfkt-007)河南中医药大学博士科研基金(BSJJ2024-04)河南省现代农业产业技术体系项目(HARS-22-11-Z3).
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