芍药苷对类风湿关节炎大鼠滑膜组织炎症的改善作用OA
The Ameliorative Effect of Paeoniflorin on Synovial Tissue Inflammation in Rats with Rheumatoid Arthritis
目的:基于PI3 K/Akt/NF-κB通路观察芍药苷对类风湿关节炎大鼠滑膜组织炎症的改善作用及其机制.方法:将 60 只SD雄性大鼠随机分为空白组、模型组、PI3 K 抑制剂组、芍药苷组、芍药苷+PI3 K激动剂组,每组 12 只.除空白组外,其余组采用Ⅱ型胶原诱导类风湿关节炎大鼠模型.观察各组大鼠不同时间点体重和关节肿胀度;采用苏木精-伊红(HE)染色和番红 O-固绿染色观察大鼠病理形态;用ELISA法检测大鼠血清中TNF-α、IL-6、IL-1β、IL-10 含量;采用Western Blot法检测大鼠滑膜组织中PI3 K、Akt、NF-κB p65、TNF-α、IL-6、IL-10 蛋白表达;采用 RT-PCR 检测大鼠滑膜组织 PI3K、Akt、NF-κB p65、TNF-α、IL-6、IL-10 mRNA 表达.结果:与模型组比较,第 21 天和第 28 天芍药苷+PI3 K激动剂组大鼠体重上升、关节肿胀度降低,差异有统计学意义(P<0.05);芍药苷组和 PI3 K 抑制剂组大鼠体重上升、关节肿胀度显著降低,差异有统计学意义(P<0.01).与模型组比较,芍药苷+PI3 K激动剂组大鼠关节软骨轻度损伤,软骨细胞增多;芍药苷组和 PI3 K 抑制剂组大鼠关节软骨损伤改善,软骨下骨结构未见明显受损.与模型组比较,芍药苷+PI3K 激动剂组大鼠血清 TNF-α、IL-6 和IL-1β水平降低,IL-10 水平升高,差异有统计学意义(P<0.05);芍药苷组和PI3 K 抑制剂组大鼠 TNF-α、IL-6 和IL-1β水平明显降低,差异有统计学意义(P<0.01);IL-10 水平显著升高,差异有统计学意义(P<0.01).Western Blot 法检测和 RT-PCR 显示,与模型组比较,芍药苷+PI3K 激动剂组大鼠PI3 K、Akt、NF-κB p65、TNF-α、IL-6 蛋白表达及基因水平降低,差异有统计学意义(P<0.05);IL-10 蛋白表达及基因水平升高,差异有统计学意义(P<0.05);芍药苷组和PI3 K抑制剂组水平变化显著,差异有统计学意义(P<0.01).结论:芍药苷可以调控PI3 K/Akt/NF-κB信号通路,从而改善类风湿关节炎大鼠滑膜组织炎症.
Objective:To observe the effect and mechanism of paeoniflorin on synovial tissue in rats with rheumatoid arthri-tis(RA),focusing based on the PI3 K/Akt/NF-κB signaling pathway.Methods:Sixty male SD rats were randomly divided into the blank group,model group,PI3 K inhibitor group,paeoniflorin group,and paeoniflorin+PI3 K agonist group,with 12 rats in each group.Except for the blank group,the other groups were established with type Ⅱ collagen-induced rat rheumatoid arthritis models.Body weight and joint swelling degree of rats in each group were monitored at different time points;pathological changes in joint tissues were assessed by hematoxylin-eosin(HE)and Safranin O-Fast Green Stai-ning.The contents of TNF-α,IL-6,IL-1β and IL-10 in rat serum were detected by ELISA.The protein expressions of PI3 K,Akt,NF-κB p65,TNF-α,IL-6 and IL-10 in the synovial tissue of rats were detected by Western Blot.The mRNA expressions levels of PI3 K,Akt,NF-κB p65,TNF-α,IL-6 and IL-10 in rat synovial tissue were quantified by RT-PCR.Results:Compared with the model group,the body weight of the paeoniflorin+PI3 K agonist group increased and the de-gree of joint swelling decreased on the 21st and 28th day(P<0.05).The body weight of the paeoniflorin group and the PI3 K inhibitor group increased,and the degree of joint swelling was significantly reduced(P<0.01).Compared with the model group,the paeoniflorin+PI3 K agonist group exhibited mild articular cartilage injury and an increased number of chondrocytes.In the paeoniflorin group and the PI3 K inhibitor group,the articular cartilage injury was improved,and no obvious damage was observed in the subchondral bone structure.Compared with the model group,the levels of serum TNF-α,IL-6 and IL-1β in the paeoniflorin+PI3 K agonist group decreased,while the level of IL-10 increased(P<0.05),and the levels of TNF-α,IL-6 and IL-1β in the paeoniflorin group and the PI3 K inhibitor group significantly decreased(P<0.01).The level of IL-10 was significantly increased(P<0.01).Western Blot and RT-PCR showed that,compared with the model group,the paeoniflorin+PI3 K agonist group exhibited decreased protein expressions and gene levels of PI3 K,Akt,NF-κB p65,TNF-α,and IL-6 decreased(P<0.05),while the protein expression and gene level of IL-10 increased(P<0.05).The levels in the paeoniflorin group and the PI3 K inhibitor group changed significantly(P<0.0 1).Conclu-sion:Paeoniflorin can regulate the PI3 K/Akt/NF-κB signaling pathway,thereby improving synovial tissue inflammation in rats with rheumatoid arthritis.
毛珍;江润;张涛;刘健;王龙
武汉市蔡甸区人民医院(武汉,430100)武汉市蔡甸区人民医院(武汉,430100)武汉市蔡甸区人民医院(武汉,430100)武汉市蔡甸区人民医院(武汉,430100)武汉市第一医院
医药卫生
芍药苷类风湿关节炎滑膜组织磷酸肌醇激酶/蛋白激酶/核转录因子-κB
paeoniflorinrheumatoid arthritissynovial tissuePI3 K/Akt/NF-κB
《中国中医骨伤科杂志》 2026 (4)
14-21,8
武汉市卫生计生科研基金资助项目(WZ24Q02)武汉市蔡甸区人民医院院内科研项目(XHJBYY-KXYJJJ-QNXM-05,XHJBKXYJJJ-MSXM-03)
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